A tetratricopeptide repeat domain-containing protein SSR1 located in mitochondria is involved in root development and auxin polar transport in Arabidopsis

doi:10.1111/tpj.12911

	A tetratricopeptide repeat domain-containing protein SSR1 located in mitochondria is involved in root development and auxin polar transport in Arabidopsis
	Zhang, Min ; Wang, Cuiping ; Lin, Qingfang ; Liu, Aihua 1; Wang, Ting ; Feng, Xuanjun ; Liu, Jie ; Han, Huiling ; Ma, Yan ; Bonea, Diana 2; Zhao, Rongmin 2; Hua, Xuejun
	2015
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	83 期号:4 页码:582-599
摘要	Auxin polar transport mediated by a group of Pin-formed (PIN) transporters plays important roles in plant root development. However, the mechanism underlying the PIN expression and targeting in response to different developmental and environmental stimuli is still not fully understood. Here, we report a previously uncharacterized gene SSR1, which encodes a mitochondrial protein with tetratricopeptide repeat (TPR) domains, and show its function in root development in Arabidopsis thaliana. In ssr1-2, a SSR1 knock-out mutant, the primary root growth was dramatically inhibited due to severely impaired cell proliferation and cell elongation. Significantly lowered level of auxin was found in ssr1-2 roots by auxin measurement and was further supported by reduced expression of DR5-driven reporter gene. As a result, the maintenance of the root stem cell niche is compromised in ssr1-2. It is further revealed that the expression level of several PIN proteins, namely, PIN1, PIN2, PIN3, PIN4 and PIN7, were markedly reduced in ssr1-2 roots. In particular, we showed that the reduced protein level of PIN2 on cell membrane in ssr1-2 is due to impaired retrograde trafficking, possibly resulting from a defect in retromer sorting system, which destines PIN2 for degradation in vacuoles. In conclusion, our results indicated that SSR1 is functioning in root development in Arabidopsis, possibly by affecting PIN protein expression and subcellular targeting. Significance StatementSSR1, a previous uncharacterized mitochondrial protein, is involved in Arabidopsis root development, possibly by affecting proper PIN protein expression and subcellular targeting.
关键词	SSR1 root development auxin transport PIN protein mitochondria
学科领域	Plant Sciences
DOI	10.1111/tpj.12911
收录类别	SCI
语种	英语
WOS关键词	STEM-CELL NICHES ; GROWTH ; SCARECROW ; PIN2 ; GRAVITROPISM ; MAINTENANCE ; TRAFFICKING ; HOMEOSTASIS ; REGULATOR ; INTERACTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000359356300002
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	National Nature Science Foundation of China [31170242] ; National Project for Transgenic Research [2014ZX08009-003]
作者邮箱	xjhua@ibcas.ac.cn ; xjhua@ibcas.ac.cn
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25846
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Manitoba, Dept Plant Sci, Winnipeg, MB R3T 2N2, Canada 3.Univ Toronto, Dept Biol Sci, Toronto, ON M1C 1A4, Canada
推荐引用方式 GB/T 7714	Zhang, Min,Wang, Cuiping,Lin, Qingfang,et al. A tetratricopeptide repeat domain-containing protein SSR1 located in mitochondria is involved in root development and auxin polar transport in Arabidopsis[J]. PLANT JOURNAL,2015,83(4):582-599.
APA	Zhang, Min.,Wang, Cuiping.,Lin, Qingfang.,Liu, Aihua.,Wang, Ting.,...&Hua, Xuejun.(2015).A tetratricopeptide repeat domain-containing protein SSR1 located in mitochondria is involved in root development and auxin polar transport in Arabidopsis.PLANT JOURNAL,83(4),582-599.
MLA	Zhang, Min,et al."A tetratricopeptide repeat domain-containing protein SSR1 located in mitochondria is involved in root development and auxin polar transport in Arabidopsis".PLANT JOURNAL 83.4(2015):582-599.

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