Virus-induced gene silencing reveals control of reactive oxygen species accumulation and salt tolerance in tomato by gamma-aminobutyric acid metabolic pathway

doi:10.1111/pce.12419

	Virus-induced gene silencing reveals control of reactive oxygen species accumulation and salt tolerance in tomato by gamma-aminobutyric acid metabolic pathway
	Bao, Hexigeduleng; Chen, Xianyang; Lv, Sulian; Jiang, Ping; Feng, Juanjuan; Fan, Pengxiang; Nie, Lingling; Li, Yinxin
	2015
发表期刊	PLANT CELL AND ENVIRONMENT
ISSN	0140-7791
卷号	38 期号:3 页码:600-613
摘要	gamma-Aminobutyric acid (GABA) accumulates in many plant species in response to environmental stress. However, the physiological function of GABA or its metabolic pathway (GABA shunt) in plants remains largely unclear. Here, the genes, including glutamate decarboxylases (SlGADs), GABAtransaminases (SlGABA-Ts) and succinic semialdehyde dehydrogenase (SlSSADH), controlling three steps of the metabolic pathway of GABA, were studied through virus-induced gene silencing approach in tomato. Silencing of SlGADs (GABA biosynthetic genes) and SlGABA-Ts (GABA catabolic genes) led to increased accumulation of reactive oxygen species (ROS) as well as salt sensitivity under 200mm NaCl treatment. Targeted quantitative analysis of metabolites revealed that GABA decreased and increased in the SlGADs- and SlGABA-Ts-silenced plants, respectively, whereas succinate (the final product of GABA metabolism) decreased in both silenced plants. Contrarily, SlSSADH-silenced plants, also defective in GABA degradation process, showed dwarf phenotype, curled leaves and enhanced accumulation of ROS in normal conditions, suggesting the involvement of a bypath for succinic semialdehyde catabolism to -hydroxybutyrate as reported previously in Arabidopsis, were less sensitive to salt stress. These results suggest that GABA shunt is involved in salt tolerance of tomato, probably by affecting the homeostasis of metabolites such as succinate and -hydroxybutyrate and subsequent ROS accumulation under salt stress. -Aminobutyric acid (GABA) accumulates in many plant species in response to environmental stress, but the physiological function of GABA or its metabolic pathway (GABA shunt) in plants remains largely unclear. In the present study, based on loss-of-function studies, our findings revealed the functional involvement of GABA shunt in the salt tolerance of tomato and the putative roles for GABA-related metabolites (such as succinate and -hydroxybutyrate) in these processes. These results open exciting perspectives for further investigations of GABA shunt and associated metabolic pathways to the stress adaptation of plants, pointing to these pathways as potential targets for engineering of plant stress tolerance. To our knowledge, this work represents one of the most thorough studies demonstrating the roles of GABA metabolic pathway in defense against environmental stress.
关键词	Solanum lycopersicum VIGS GABA ROS abiotic stress electron transport chain TCA cycle
学科领域	Plant Sciences
DOI	10.1111/pce.12419
收录类别	SCI
语种	英语
WOS关键词	SUCCINIC-SEMIALDEHYDE-DEHYDROGENASE ; GLUTAMATE-DECARBOXYLASE ; OXIDATIVE STRESS ; SALINITY TOLERANCE ; GABA TRANSAMINASE ; MITOCHONDRIAL RESPIRATION ; SUBCELLULAR-LOCALIZATION ; NICOTIANA-BENTHAMIANA ; CARBON METABOLISM ; PLANT RESPIRATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000349994600019
出版者	WILEY-BLACKWELL
文献子类	Article
出版地	HOBOKEN
EISSN	1365-3040
资助机构	National High Technology and Research Development Program of China ('863' project) [2007AA091705] ; Chinese Ministry of Agriculture [2013ZX08009-003-002]
作者邮箱	yxli@ibcas.ac.cn
引用统计	被引频次：70[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25854
专题	中科院植物分子生理学重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Bao, Hexigeduleng,Chen, Xianyang,Lv, Sulian,et al. Virus-induced gene silencing reveals control of reactive oxygen species accumulation and salt tolerance in tomato by gamma-aminobutyric acid metabolic pathway[J]. PLANT CELL AND ENVIRONMENT,2015,38(3):600-613.
APA	Bao, Hexigeduleng.,Chen, Xianyang.,Lv, Sulian.,Jiang, Ping.,Feng, Juanjuan.,...&Li, Yinxin.(2015).Virus-induced gene silencing reveals control of reactive oxygen species accumulation and salt tolerance in tomato by gamma-aminobutyric acid metabolic pathway.PLANT CELL AND ENVIRONMENT,38(3),600-613.
MLA	Bao, Hexigeduleng,et al."Virus-induced gene silencing reveals control of reactive oxygen species accumulation and salt tolerance in tomato by gamma-aminobutyric acid metabolic pathway".PLANT CELL AND ENVIRONMENT 38.3(2015):600-613.

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