Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans

doi:10.1007/s11120-014-0039-z

IB-CAS > 中科院光生物学重点实验室

	Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans
	Qin, Xiaochun ; Wang, Wenda ; Chang, Lijing 1; Chen, Jinghua ; Wang, Peng 2; Zhang, Jianping 2; He, Yikun 1; Kuang, Tingyun ; Shen, Jian-Ren
	2015
发表期刊	PHOTOSYNTHESIS RESEARCH
ISSN	0166-8595
卷号	123 期号:1 页码:61-76
摘要	A novel super-complex of photosystem I (PSI)-light-harvesting complex I (LHCI) was isolated from a siphonaceous marine green alga, Bryopsis corticulans. The super-complex contained 9-10 Lhca antennas as external LHCI bound to the core complex. The super-complex was further disintegrated into PSI core and LHCI sub-complexes, and analysis of the pigment compositions by high-performance liquid chromatography revealed unique characteristics of the B. corticulans PSI in that one PSI core contained around 14 a-carotenes and 1-2 e-carotenes. This is in sharp contrast to the PSI core from higher plants and most cyanobacteria where only beta-carotenes were present, and is the first report for an alpha-carotenetype PSI core complex among photosynthetic eukaryotes, suggesting a structural flexibility of the PSI core. Lhca antennas from B. corticulans contained seven kinds of carotenoids (siphonaxanthin, all-trans neoxanthin, 9'-cis neoxanthin, violaxanthin, siphonein, e-carotene, and acarotene) and showed a high carotenoid: chlorophyll ratio of around 7.5: 13. PSI-LHCI super-complex and PSI core showed fluorescence emission peaks at 716 and 718 nm at 77 K, respectively; whereas two Lhca oligomers had fluorescence peaks at 681 and 684 nm, respectively. By comparison with spinach PSI preparations, it was found that B. corticulans PSI had less red chlorophylls, most of them are present in the core complex but not in the outer light-harvesting systems. These characteristics may contribute to the fine tuning of the energy transfer network, and to acclimate to the ever-changing light conditions under which the unique green alga inhabits.
关键词	Bryopsis corticulans alpha-Carotene Siphonaxanthin Siphonein Neoxanthin Photosystem I
学科领域	Plant Sciences
DOI	10.1007/s11120-014-0039-z
收录类别	SCI
语种	英语
WOS关键词	LIGHT-HARVESTING-COMPLEX ; PLANT PHOTOSYSTEM-I ; CHLOROPHYLL-PROTEIN COMPLEXES ; CHLAMYDOMONAS-REINHARDTII ; ANGSTROM RESOLUTION ; ENERGY-TRANSFER ; SUPRAMOLECULAR ORGANIZATION ; STARK SPECTROSCOPY ; CRYSTAL-STRUCTURE ; BINDING-PROTEINS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000348379100005
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5079
资助机构	National Basic Research Program of China (973 Program) [2011CBA00901] ; National Natural Science Foundation [30900095]
作者邮箱	qinxiaochun@ibcas.ac.cn ; kuangty@ibcas.ac.cn ; shen@cc.okayama-u.ac.jp
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25857
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China 3.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China 4.Okayama Univ, Grad Sch Nat Sci & Technol, Photosynth Res Ctr, Okayama 7008530, Japan
推荐引用方式 GB/T 7714	Qin, Xiaochun,Wang, Wenda,Chang, Lijing,et al. Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans[J]. PHOTOSYNTHESIS RESEARCH,2015,123(1):61-76.
APA	Qin, Xiaochun.,Wang, Wenda.,Chang, Lijing.,Chen, Jinghua.,Wang, Peng.,...&Shen, Jian-Ren.(2015).Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans.PHOTOSYNTHESIS RESEARCH,123(1),61-76.
MLA	Qin, Xiaochun,et al."Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans".PHOTOSYNTHESIS RESEARCH 123.1(2015):61-76.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
25214185.pdf（10644KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文