Genomic Characterization Reveals Insights Into Patulin Biosynthesis and Pathogenicity in Penicillium Species

doi:10.1094/MPMI-12-14-0398-FI

	Genomic Characterization Reveals Insights Into Patulin Biosynthesis and Pathogenicity in Penicillium Species
	Li, Boqiang ; Zong, Yuanyuan ; Du, Zhenglin 1; Chen, Yong ; Zhang, Zhanquan ; Qin, Guozheng; Zhao, Wenming 1; Tian, Shiping
	2015
发表期刊	MOLECULAR PLANT-MICROBE INTERACTIONS
ISSN	0894-0282
卷号	28 期号:6 页码:635-647
摘要	Penicillium species are fungal pathogens that infect crop plants worldwide. P. expansum differs from P. italicum and P. digitatum, all major postharvest pathogens of pome and citrus, in that the former is able to produce the mycotoxin patulin and has a broader host range. The molecular basis of host-specificity of fungal pathogens has now become the focus of recent research. The present report provides the whole genome sequence of P. expansum (33.52 Mb) and P. italicum (28.99 Mb) and identifies differences in genome structure, important pathogenic characters, and secondary metabolite (SM) gene clusters in Penicillium species. We identified a total of 55 gene clusters potentially related to secondary metabolism, including a cluster of 15 genes (named PePatA to PePatO), that may be involved in patulin biosynthesis in P. expansum. Functional studies confirmed that PePatL and PePatK play crucial roles in the biosynthesis of patulin and that patulin production is not related to virulence of P. expansum. Collectively, P. expansum contains more pathogenic genes and SM gene clusters, in particular, an intact patulin cluster, than P. italicum or P. digitatum. These findings provide important information relevant to understanding the molecular network of patulin biosynthesis and mechanisms of host-specificity in Penicillium species.
学科领域	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences
DOI	10.1094/MPMI-12-14-0398-FI
收录类别	SCI
语种	英语
WOS关键词	CARBOHYDRATE-ACTIVE ENZYMES ; SECONDARY METABOLISM ; FILAMENTOUS FUNGI ; GENE-CLUSTER ; EXPANSUM ; ALIGNMENT ; ASPERGILLUS ; SEQUENCE ; DATABASE ; DIVERSIFICATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000356953600001
出版者	AMER PHYTOPATHOLOGICAL SOC
文献子类	Article
出版地	ST PAUL
EISSN	1943-7706
资助机构	National High Technology Research (863) Program of China [2012AA101607] ; National Natural Science Foundation of China [31030051] ; Chinese Academy of Sciences [KSCX2-EW-G-6]
作者邮箱	tsp@ibcas.ac.cn
作品OA属性	hybrid
引用统计	被引频次：125[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25862
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Beijing Inst Genom, Core Genom Facil, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Boqiang,Zong, Yuanyuan,Du, Zhenglin,et al. Genomic Characterization Reveals Insights Into Patulin Biosynthesis and Pathogenicity in Penicillium Species[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2015,28(6):635-647.
APA	Li, Boqiang.,Zong, Yuanyuan.,Du, Zhenglin.,Chen, Yong.,Zhang, Zhanquan.,...&Tian, Shiping.(2015).Genomic Characterization Reveals Insights Into Patulin Biosynthesis and Pathogenicity in Penicillium Species.MOLECULAR PLANT-MICROBE INTERACTIONS,28(6),635-647.
MLA	Li, Boqiang,et al."Genomic Characterization Reveals Insights Into Patulin Biosynthesis and Pathogenicity in Penicillium Species".MOLECULAR PLANT-MICROBE INTERACTIONS 28.6(2015):635-647.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
mpmi-12-14-0398-fi.p（2688KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文