Relationships between denitrification gene expression, dissimilatory nitrate reduction to ammonium and nitrous oxide and dinitrogen production in montane grassland soils

doi:10.1016/j.soilbio.2015.03.030

	Relationships between denitrification gene expression, dissimilatory nitrate reduction to ammonium and nitrous oxide and dinitrogen production in montane grassland soils
	Chen, Zhe 1; Wang, Changhui 2; Gschwendtner, Silvia 3; Willibald, Georg ; Unteregelsbacher, Sebastian ; Lu, Haiyan ; Kolar, Allison ; Schloter, Michael 3; Butterbach-Bahl, Klaus ; Dannenmann, Michael
	2015
发表期刊	SOIL BIOLOGY & BIOCHEMISTRY
ISSN	0038-0717
卷号	87 页码:67-77
摘要	The montane grassland soils of Europe store significant amounts of nitrogen (N), and climate change might drive their volatilization due to the stimulation of gaseous nitrous oxide (N2O) and dinitrogen (N-2) losses. Hence, a thorough, mechanistic understanding of the processes responsible for N loss and retention such as denitrification and dissimilatory nitrate reduction to ammonium (DNRA) in these soils is urgently needed. Here we aimed to explore the relationships between denitrifier gene abundance and expression with N-2 and N2O production and the importance of DNRA versus denitrification in nitrate consumption and N2O production for typical montane grassland soils of Southern Germany. In a laboratory incubation experiment with glucose and nitrate addition, we combined direct measurements of N2O and N-2 production with a molecular analysis of the denitrifier communities involved in nitrite, nitric oxide (NO) and N2O reduction and with the quantification of DNRA. The soils originated from a space-for-time climate change experiment, where intact plant-soil mesocosms were exposed for three years either to ambient conditions at a high elevation site (HE control treatment) or to predicted climate change conditions (warming, reduced summer precipitation and reduced winter snow cover) by translocation to lower elevation (LE climate change treatment). The abundance (DNA) of cnorB genes was significantly reduced in LE soils, whereas the abundance of nosZ genes did not differ between the HE and LE soils. However, the decreased abundance of cnorB genes unexpectedly resulted in slightly increased rather than decreased potential N2O emissions. This effect could be explained by the increased levels of cnorB mRNA and, therefore, the higher physiological activity of the NO reducers in the LE soils. In contrast with the DNA levels, the dynamics of the cnorB mRNA levels followed N2O emission patterns, whereas the nosZ expression was strongly correlated with the N-2 emission (R-2 = 0.83). The potential rates of DNRA were approximately one-third of the rates of denitrification, and DNRA was not a source for N2O. We conclude that DNRA significantly competes with denitrification in these soils, thus contributing to N conservation. This work demonstrates that the molecular analysis of nosZ gene expression has great potential to contribute to solving the enigmatic problem of understanding N-2 loss from soil. (C) 2015 Elsevier Ltd. All rights reserved.
关键词	N2O and N-2 cnorB and nosZ Denitrification DNRA Climate change Montane grassland
学科领域	Soil Science
DOI	10.1016/j.soilbio.2015.03.030
收录类别	SCI
语种	英语
WOS关键词	MESSENGER-RNA LEVELS ; DENITRIFYING BACTERIA ; ACETYLENE INHIBITION ; MICROBIAL COMMUNITY ; RIBOSOMAL-RNA ; N2O EMISSIONS ; NOSZ GENES ; ABUNDANCE ; NIRK ; QUANTIFICATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000357244300008
出版者	PERGAMON-ELSEVIER SCIENCE LTD
文献子类	Article
出版地	OXFORD
EISSN	1879-3428
资助机构	Alexander von Humboldt Foundation [1148319] ; terrestrial ecosystem observatory initiative (TERENO) by BMBF ; Helmholtz Society ; Bavarian government within the FORKAST project ; National Natural Science Foundation of China [21107109] ; German Science Foundation (DFG) [DA 1217/2-1, SCHL 446/11-1]
作者邮箱	michael.dannenmann@kit.edu
引用统计	被引频次：57[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25874
专题	植被与环境变化国家重点实验室
作者单位	1.Karlsruhe Inst Technol, Inst Meteorol & Climate Res, Atmospher Environm Res IMK IFU, D-82467 Garmisch Partenkirchen, Germany 2.Chinese Acad Sci, Inst Atmospher Phys, State Key Lab Atmospher Boundary Layer Phys & Atm, Beijing 100029, Peoples R China 3.Chinese Acad Sci IBCAS, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 4.German Res Ctr Environm Hlth GmbH, Res Unit Environm Genom, D-85764 Neuherberg, Germany
推荐引用方式 GB/T 7714	Chen, Zhe,Wang, Changhui,Gschwendtner, Silvia,et al. Relationships between denitrification gene expression, dissimilatory nitrate reduction to ammonium and nitrous oxide and dinitrogen production in montane grassland soils[J]. SOIL BIOLOGY & BIOCHEMISTRY,2015,87:67-77.
APA	Chen, Zhe.,Wang, Changhui.,Gschwendtner, Silvia.,Willibald, Georg.,Unteregelsbacher, Sebastian.,...&Dannenmann, Michael.(2015).Relationships between denitrification gene expression, dissimilatory nitrate reduction to ammonium and nitrous oxide and dinitrogen production in montane grassland soils.SOIL BIOLOGY & BIOCHEMISTRY,87,67-77.
MLA	Chen, Zhe,et al."Relationships between denitrification gene expression, dissimilatory nitrate reduction to ammonium and nitrous oxide and dinitrogen production in montane grassland soils".SOIL BIOLOGY & BIOCHEMISTRY 87(2015):67-77.

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