Effects of nitrogen deposition rates and frequencies on the abundance of soil nitrogen-related functional genes in temperate grassland of northern China

doi:10.1007/s11368-015-1061-2

	Effects of nitrogen deposition rates and frequencies on the abundance of soil nitrogen-related functional genes in temperate grassland of northern China
	Ning, Qiushi 1,5; Gu, Qian 1; Shen, Jupei 4; Lv, Xiaotao 2; Yang, Junjie 2; Zhang, Ximei 2; He, Jizheng 4,5; Huang, Jianhui; Wang, Hong 3; Xu, Zhihong 5; Han, Xingguo2
	2015
发表期刊	JOURNAL OF SOILS AND SEDIMENTS
ISSN	1439-0108
卷号	15 期号:3 页码:694-704
摘要	Microbial processes driving nitrogen (N) cycling are hot topics in terms of increasing N deposition. Abundances of N-related functional genes (NFG) can be most responsive to N deposition and commonly used to represent N transformation rates. However, empirically simulated N deposition has been exclusively conducted through large and infrequent N fertilization, which may have caused contrasting effects on NFGs. Therefore, experiments with small and frequent N additions closed to natural deposition are necessary. Independently manipulated N addition rates (i.e., 0, 5, 10, 15, 20, and 50 g N m(-2) year(-1)) and two frequencies (2 times per year addition as conventional large and infrequent N fertilization (2 N), and 12 times per year addition simulating small and frequent N deposition (12 N), respectively) were conducted in a long-term field experiment of a semiarid grassland in northern China. Quantification analysis using real-time PCR were carried out for NFGs, including nifH for N fixation, chiA for N mineralization, archaeal (AOA) and bacterial (AOB) amoA for nitrification, and narG, nirS, nirK, and nosZ for denitrification. NFG abundances showed distinct sensitivities to N addition rates. The nifH, AOA-amoA, nirS, and nosZ gene abundances increased due to improved available N at low N rates, but suppressed by salt toxicity and acidification at high N rates. Large changes of chiA and AOB-amoA gene abundances highlighted their great sensitivities to the N enrichment. The abundance of AOB-amoA was more sensitive to N addition than AOA-amoA, but AOA-amoA dominated in absolute numbers and they predominated the ammonia-oxidation under different conditions. The N addition frequencies caused significant lower gene abundances of nifH, nirS, and nosZ under the 2-N frequency due to stronger suppression of acidification and salt toxicity and resulted in significant higher AOB-amoA gene abundances in response to higher N availability under the 2-N frequency. The NFGs abundances responded to N addition rates distinctly, highlighting that the driven processes involved in N cycling were altered by the N addition rates. The different effects of two N addition frequencies on NFG abundances demonstrated that conventional large and infrequent N fertilization cannot represent N deposition, and small and frequent N addition should be employed to project the effects of N deposition on microbial functional groups as well as on N transformations.
关键词	Functional gene Nitrogen addition frequency Nitrogen addition rate Nitrogen deposition Nitrogen transformation Temperate grassland
学科领域	Environmental Sciences ; Soil Science
DOI	10.1007/s11368-015-1061-2
收录类别	SCI
语种	英语
WOS关键词	AMMONIA-OXIDIZING BACTERIA ; GLOBAL CLIMATE-CHANGE ; DENITRIFYING BACTERIA ; SPECIES RICHNESS ; N DEPOSITION ; CYCLE GENES ; NOSZ GENES ; ARCHAEA ; COMMUNITIES ; DENITRIFICATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000349605100019
出版者	SPRINGER HEIDELBERG
文献子类	Article
出版地	HEIDELBERG
EISSN	1614-7480
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences [XDB15010401, XDB15010403] ; National Natural Science Foundation of China [31170433, 31307337]
作者邮箱	zhihong.xu@griffith.edu.au ; xghan@ibcas.ac.cn
作品OA属性	Green Submitted
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25876
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110164, Peoples R China 4.Agr & Agri Food Canada, Semiarid Prairie Agr Res Ctr, Swift Current, SK S9H 3X2, Canada 5.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China 6.Griffith Univ, Environm Futures Res Inst, Nathan, Qld 4111, Australia
推荐引用方式 GB/T 7714	Ning, Qiushi,Gu, Qian,Shen, Jupei,et al. Effects of nitrogen deposition rates and frequencies on the abundance of soil nitrogen-related functional genes in temperate grassland of northern China[J]. JOURNAL OF SOILS AND SEDIMENTS,2015,15(3):694-704.
APA	Ning, Qiushi.,Gu, Qian.,Shen, Jupei.,Lv, Xiaotao.,Yang, Junjie.,...&Han, Xingguo.(2015).Effects of nitrogen deposition rates and frequencies on the abundance of soil nitrogen-related functional genes in temperate grassland of northern China.JOURNAL OF SOILS AND SEDIMENTS,15(3),694-704.
MLA	Ning, Qiushi,et al."Effects of nitrogen deposition rates and frequencies on the abundance of soil nitrogen-related functional genes in temperate grassland of northern China".JOURNAL OF SOILS AND SEDIMENTS 15.3(2015):694-704.

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