Role of a chalcone isomerase-like protein in flavonoid biosynthesis in Arabidopsis thaliana

doi:10.1093/jxb/erv413

	Role of a chalcone isomerase-like protein in flavonoid biosynthesis in Arabidopsis thaliana
	Jiang, Wenbo 1; Yin, Qinggang 1,2; Wu, Ranran 1,2; Zheng, Guangshun 1,2; Liu, Jinyue 1,2; Dixon, Richard A.; Pang, Yongzhen 1
	2015
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	66 期号:22 页码:7165-7179
摘要	Flavonoids are important natural products for plant defence and human health. Although almost all the flavonoid pathway genes have been well-documented by biochemical and/or genetic approaches, the role of the Arabidopsis chalcone isomerase-like (CHIL) gene remains unclear. Two chil mutants with a seed colour similar to that of wild-type Arabidopsis have been identified here, but in sharp contrast to the characteristic transparent testa seed phenotype associated with other known flavonoid pathway genes. CHIL loss-of-function mutations led to a strong reduction in the proanthocyanidin and flavonol levels in seeds, but not in the anthocyanin levels in leaves. CHIL over-expression could partially recover the mutant phenotype of the chil mutant and increased both proanthocyanidin and flavonol accumulation in wild-type Arabidopsis. However, the CHIL gene could not rescue the mutant phenotype of TT5 that encodes the intrinsic chalcone isomerase in Arabidopsis. Parallel phenotypical and metabolic analyses of the chil, tt5, chs, and f3h mutants revealed that, genetically, CHIL functions at the same step as TT5. Moreover, it is demonstrated that CHIL co-expresses, co-localizes, and interacts with TT5 in Arabidopsis for flavonoid production. Based on these genetic and metabolic studies, it is concluded that CHIL functions with TT5 to promote flavonoid production, which is a unique enhancer in the flavonoid pathway.
关键词	Arabidopsis chalcone isomerase-like flavonoids flavonols proanthocyanidins seed coat
学科领域	Plant Sciences
DOI	10.1093/jxb/erv413
收录类别	SCI
语种	英语
WOS关键词	PROANTHOCYANIDIN BIOSYNTHESIS ; MEDICAGO-TRUNCATULA ; SEED ; GENETICS ; ELEMENTS ; MUTANTS ; TESTA ; BIOCHEMISTRY ; ANTHOCYANIN ; MUTAGENESIS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000367815000015
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	Major State Basic Research Development Program [2013CB127002] ; National Natural Science Foundation of China [31370335] ; Chinese Academy of Sciences [39391503-7]
作者邮箱	yzhpang@ibcas.ac.cn
作品OA属性	Green Published, hybrid, Green Submitted
引用统计	被引频次：103[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25880
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Dixon, Richard A.] Univ N Texas, Dept Biol Sci, Denton, TX USA
推荐引用方式 GB/T 7714	Jiang, Wenbo,Yin, Qinggang,Wu, Ranran,et al. Role of a chalcone isomerase-like protein in flavonoid biosynthesis in Arabidopsis thaliana[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(22):7165-7179.
APA	Jiang, Wenbo.,Yin, Qinggang.,Wu, Ranran.,Zheng, Guangshun.,Liu, Jinyue.,...&Pang, Yongzhen.(2015).Role of a chalcone isomerase-like protein in flavonoid biosynthesis in Arabidopsis thaliana.JOURNAL OF EXPERIMENTAL BOTANY,66(22),7165-7179.
MLA	Jiang, Wenbo,et al."Role of a chalcone isomerase-like protein in flavonoid biosynthesis in Arabidopsis thaliana".JOURNAL OF EXPERIMENTAL BOTANY 66.22(2015):7165-7179.

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