Arabidopsis NIP3;1 Plays an Important Role in Arsenic Uptake and Root-to-Shoot Translocation under Arsenite Stress Conditions

doi:10.1016/j.molp.2015.01.005

	Arabidopsis NIP3;1 Plays an Important Role in Arsenic Uptake and Root-to-Shoot Translocation under Arsenite Stress Conditions
	Xu, Wenzhong; Dai, Wentao; Yan, Huili; Li, Sheng; Shen, Hongling; Chen, Yanshan; Xu, Hua; Sun, Yangyang; He, Zhenyan; Ma, Mi
	2015
发表期刊	MOLECULAR PLANT
ISSN	1674-2052
卷号	8 期号:5 页码:722-733
摘要	In Arabidopsis, the nodulin 26-like intrinsic protein (NIP) subfamily of aquaporin proteins consists of nine members, five of which (NIP1;1, NIP1; 2, NIP5;1, NIP6;1, and NIP7;1) were previously identified to be permeable to arsenite. However, the roles of NIPs in the root-to-shoot translocation of arsenite in plants remain poorly understood. In this study, using reverse genetic strategies, Arabidopsis NIP3;1 was identified to play an important role in both the arsenic uptake and root-to-shoot distribution under arsenite stress conditions. The nip3;1 loss-of-function mutants displayed obvious improvements in arsenite tolerance for aboveground growth and accumulated less arsenic in shoots than those of the wild-type plants, whereas the nip3; 1 nip1;1 double mutant showed strong arsenite tolerance and improved growth of both roots and shoots under arsenite stress conditions. A promoter-beta-glucuronidase analysis revealed that NIP3; 1 was expressed almost exclusively in roots (with the exception of the root tips), and heterologous expression in the yeast Saccharomyces cerevisiae demonstrated that NIP3; 1 was able to mediate arsenite transport. Taken together, our results suggest that NIP3; 1 is involved in arsenite uptake and root-to-shoot translocation in Arabidopsis, probably as a passive and bidirectional arsenite transporter.
关键词	Arabidopsis arsenite NIP3 1 root-to-shoot translocation tolerance
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.molp.2015.01.005
收录类别	SCI
语种	英语
WOS关键词	RICE AQUAPORIN LSI1 ; PHYTOCHELATINS ; ACCUMULATION ; TOLERANCE ; TRANSPORT ; XYLEM ; IDENTIFICATION ; REDUCTION ; PLANTS ; HYPERACCUMULATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000353999300006
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1752-9867
资助机构	National Natural Science Foundation of China [31170164, 31070449] ; CAS [KSCX2-EW-J-1, KSCX2-YW-G053-4]
作者邮箱	mami@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：120[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25881
专题	中科院北方资源植物重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Xu, Wenzhong,Dai, Wentao,Yan, Huili,et al. Arabidopsis NIP3;1 Plays an Important Role in Arsenic Uptake and Root-to-Shoot Translocation under Arsenite Stress Conditions[J]. MOLECULAR PLANT,2015,8(5):722-733.
APA	Xu, Wenzhong.,Dai, Wentao.,Yan, Huili.,Li, Sheng.,Shen, Hongling.,...&Ma, Mi.(2015).Arabidopsis NIP3;1 Plays an Important Role in Arsenic Uptake and Root-to-Shoot Translocation under Arsenite Stress Conditions.MOLECULAR PLANT,8(5),722-733.
MLA	Xu, Wenzhong,et al."Arabidopsis NIP3;1 Plays an Important Role in Arsenic Uptake and Root-to-Shoot Translocation under Arsenite Stress Conditions".MOLECULAR PLANT 8.5(2015):722-733.

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