Phosphorylation of Serine 186 of bHLH Transcription Factor SPEECHLESS Promotes Stomatal Development in Arabidopsis

doi:10.1016/j.molp.2014.12.014

	Phosphorylation of Serine 186 of bHLH Transcription Factor SPEECHLESS Promotes Stomatal Development in Arabidopsis
	Yang, Ke-Zhen ; Jiang, Min ; Wang, Ming ; Xue, Shan ; Zhu, Ling-Ling ; Wang, Hong-Zhe ; Zou, Jun-Jie ; Lee, Eun-Kyoung ; Sack, Fred 9; Le, Jie7
	2015
发表期刊	MOLECULAR PLANT
ISSN	1674-2052
卷号	8 期号:5 页码:783-795
摘要	The initiation of stomatal lineage and subsequent asymmetric divisions in Arabidopsis require the activity of the basic helix-loop-helix transcription factor SPEECHLESS (SPCH). It has been shown that SPCH controls entry into the stomatal lineage as a substrate either of the MITOGEN-ACTIVATED PROTEIN KINASE (MAPK) cascade or GSK3-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2). Here we show that three serine residues of SPCH appear to be the primary phosphorylation targets of Cyclin-Dependent Kinases A; 1 (CDKA; 1) in vitro, and among them Serine 186 plays a crucial role in stomatal formation. Expression of an SPCH construct harboring a mutation that results in phosphorylation deficiencies on Serine 186 residue failed to rescue stomatal defects in spch null mutants. Expression of a phosphorylation-mimic mutant SPCHS186D complemented stomatal production defects in the transgenic lines harboring the targeted expression of dominant-negative CDKA; 1.N146. Therefore, in addition to MAPK-and BIN2-mediated phosphorylation on SPCH, phosphorylation at Serine 186 is positively required for SPCH function in regulating stomatal development.
关键词	Arabidopsis stomata development transcription factor phosphorylation
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.molp.2014.12.014
收录类别	SCI
语种	英语
WOS关键词	CYCLIN-DEPENDENT-KINASE ; ASYMMETRIC CELL DIVISIONS ; 4 LIPS ; LINEAGE ; DIFFERENTIATION ; PROLIFERATION ; EXPRESSION ; INITIATION ; COMPLEXES ; MUTANTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000353999300010
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1752-9867
资助机构	National Natural Science Foundation of China [31271463, 31471362, 31071198, 31471285] ; Chinese Academy of Sciences [KSCX2-YW-N-073] ; Natural Sciences and Engineering Research Council of Canada
作者邮箱	lejie@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：39[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25892
专题	中科院植物分子生理学重点实验室
作者单位	1.[Yang, Ke-Zhen 2.Jiang, Min 3.Wang, Ming 4.Xue, Shan 5.Zhu, Ling-Ling 6.Wang, Hong-Zhe 7.Zou, Jun-Jie 8.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 9.[Lee, Eun-Kyoung 10.Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada
推荐引用方式 GB/T 7714	Yang, Ke-Zhen,Jiang, Min,Wang, Ming,et al. Phosphorylation of Serine 186 of bHLH Transcription Factor SPEECHLESS Promotes Stomatal Development in Arabidopsis[J]. MOLECULAR PLANT,2015,8(5):783-795.
APA	Yang, Ke-Zhen.,Jiang, Min.,Wang, Ming.,Xue, Shan.,Zhu, Ling-Ling.,...&Le, Jie.(2015).Phosphorylation of Serine 186 of bHLH Transcription Factor SPEECHLESS Promotes Stomatal Development in Arabidopsis.MOLECULAR PLANT,8(5),783-795.
MLA	Yang, Ke-Zhen,et al."Phosphorylation of Serine 186 of bHLH Transcription Factor SPEECHLESS Promotes Stomatal Development in Arabidopsis".MOLECULAR PLANT 8.5(2015):783-795.

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