Range-wide multilocus phylogenetic analyses of Pedicularis sect. Cyathophora (Orobanchaceae): Implications for species delimitation and speciation

doi:10.12705/645.6

	Range-wide multilocus phylogenetic analyses of Pedicularis sect. Cyathophora (Orobanchaceae): Implications for species delimitation and speciation
	Wang, Hong-Juan ; Li, Wei-Tao ; Liu, Ya-Nan ; Yang, Fu-Sheng ; Wang, Xiao-Quan
	2015
发表期刊	TAXON
ISSN	0040-0262
卷号	64 期号:5 页码:959-974
摘要	Accurate species delimitation is crucial to the fields of systematics, biogeography, and conservation biology; however, inferring phylogenetic relationships among closely related, recently diverged species has always been difficult. In the present study, we conducted a range-wide field investigation of five Pedicularis species of P. sect. Cyathophora, which includes all four basic corolla types present in the genus. We reconstructed the phylogeny of this section by using three single/low-copy nuclear genes (CRC, LFY-L, LFY-S) and two chloroplast genes (matK, ycf1). To clarify the evolutionary history of sect. Cyathophora, we also estimated divergence times of the genus Pedicularis. Additionally, we evaluated the phylogenetic utility of nrDNA ITS in sect. Cyathophora by cloning and sequencing analyses of the ITS region for all of the investigated samples. The results of our multilocus phylogenetic analysis supported the delimitation of three species of sect. Cyathophora, but failed to support the definition of P. rex and P. thamnophila. On the basis of morphological, geographic, genetic, and phylogenetic evidence, we suggest to include P. thamnophila in P. rex. The molecular dating analysis showed that the uplift of the Qinghai-Tibet Plateau and climatic changes may have played important roles in the divergence and speciation of sect. Cyathophora. The results of clone-based sequencing revealed that extensive nrITS polymorphisms exist in the individuals of ingroups; these polymorphisms may cause misleading phylogenetic inference for sect. Cyathophora.
关键词	molecular dating molecular phylogeny Pedicularis sect. Cyathophora single/low-copy nuclear genes species delimitation
学科领域	Plant Sciences ; Evolutionary Biology
DOI	10.12705/645.6
收录类别	SCI
语种	英语
WOS关键词	YABBY GENE FAMILY ; LONGIFLORA OROBANCHACEAE ; POLLINATION ECOLOGY ; INFERRING PHYLOGENY ; DIVERGENCE TIMES ; TIBETAN PLATEAU ; 2ND INTRON ; CRABS-CLAW ; EVOLUTION ; SEQUENCES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000363884000006
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1996-8175
资助机构	National Natural Science Foundation of China [31330008, 31110103911] ; Chinese Academy of Sciences [KJZD-EW-L07] ; Science and Technology Basic Work [2013FY112100]
作者邮箱	yang_fsh@ibcas.ac.cn ; xiaoq_wang@ibcas.ac.cn
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25921
专题	系统与进化植物学国家重点实验室
作者单位	1.[Wang, Hong-Juan 2.Li, Wei-Tao 3.Liu, Ya-Nan 4.Yang, Fu-Sheng 5.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Hong-Juan,Li, Wei-Tao,Liu, Ya-Nan,et al. Range-wide multilocus phylogenetic analyses of Pedicularis sect. Cyathophora (Orobanchaceae): Implications for species delimitation and speciation[J]. TAXON,2015,64(5):959-974.
APA	Wang, Hong-Juan,Li, Wei-Tao,Liu, Ya-Nan,Yang, Fu-Sheng,&Wang, Xiao-Quan.(2015).Range-wide multilocus phylogenetic analyses of Pedicularis sect. Cyathophora (Orobanchaceae): Implications for species delimitation and speciation.TAXON,64(5),959-974.
MLA	Wang, Hong-Juan,et al."Range-wide multilocus phylogenetic analyses of Pedicularis sect. Cyathophora (Orobanchaceae): Implications for species delimitation and speciation".TAXON 64.5(2015):959-974.

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