Proteomic analysis of endoplasmic reticulum stress responses in rice seeds

doi:10.1038/srep14255

	Proteomic analysis of endoplasmic reticulum stress responses in rice seeds
	Qian, Dandan; Tian, Lihong; Qu, Leqing
	2015
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	5
摘要	The defects in storage proteins secretion in the endosperm of transgenic rice seeds often leads to endoplasmic reticulum (ER) stress, which produces floury and shrunken seeds, but the mechanism of this response remains unclear. We used an iTRAQ-based proteomics analysis of ER-stressed rice seeds due to the endosperm-specific suppression of OsSar1 to identify changes in the protein levels in response to ER stress. ER stress changed the expression of 405 proteins in rice seed by > 2.0-fold compared with the wild-type control. Of these proteins, 140 were upregulated and 265 were downregulated. The upregulated proteins were mainly involved in protein modification, transport and degradation, and the downregulated proteins were mainly involved in metabolism and stress/defense responses. A KOBAS analysis revealed that protein-processing in the ER and degradation-related proteasome were the predominant upregulated pathways in the rice endosperm in response to ER stress. Trans-Golgi protein transport was also involved in the ER stress response. Combined with bioinformatic and molecular biology analyses, our proteomic data will facilitate our understanding of the systemic responses to ER stress in rice seeds.
学科领域	Multidisciplinary Sciences
DOI	10.1038/srep14255
收录类别	SCI
语种	英语
WOS关键词	UNFOLDED PROTEIN RESPONSE ; TRANSCRIPTION FACTOR ; ER STRESS ; QUALITY-CONTROL ; ENDOSPERM CELLS ; GENE-EXPRESSION ; MESSENGER-RNA ; ARABIDOPSIS ; INVOLVEMENT ; PROGLUTELIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000361592700001
出版者	NATURE PUBLISHING GROUP
文献子类	Article
出版地	LONDON
资助机构	Natural Science Foundation of China [31171368, 31300212]
作者邮箱	lqqu@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：38[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25929
专题	中科院植物分子生理学重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Qian, Dandan,Tian, Lihong,Qu, Leqing. Proteomic analysis of endoplasmic reticulum stress responses in rice seeds[J]. SCIENTIFIC REPORTS,2015,5.
APA	Qian, Dandan,Tian, Lihong,&Qu, Leqing.(2015).Proteomic analysis of endoplasmic reticulum stress responses in rice seeds.SCIENTIFIC REPORTS,5.
MLA	Qian, Dandan,et al."Proteomic analysis of endoplasmic reticulum stress responses in rice seeds".SCIENTIFIC REPORTS 5(2015).

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