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Crystal structure of the catalytic subunit of magnesium chelatase | |
Chen, Xuemin1; Pu, Hua1; Fang, Ying1; Wang, Xiao1; Zhao, Shun1; Lin, Yajing2; Zhang, Min3; Dai, Huai-En; Gong, Weimin2,4; Liu, Lin | |
2015 | |
发表期刊 | NATURE PLANTS |
ISSN | 2055-026X |
卷号 | 1期号:9 |
摘要 | Tetrapyrroles, including haem and chlorophyll, play vital roles for various biological processes, such as respiration and photosynthesis, and their biosynthesis is critical for virtually all organisms. In photosynthetic organisms, magnesium chelatase (MgCh) catalyses insertion of magnesium into the centre of protoporphyrin IX, the branch-point precursor for both haem and chlorophyll, leading tetrapyrrole biosynthesis into the magnesium branch1,2. This reaction needs a cooperated action of the three subunits of MgCh: the catalytic subunit ChlH and two AAA(+) subunits, ChlI and ChlD ( refs 3-5). To date, the mechanism of MgCh awaits further elucidation due to a lack of high-resolution structures, especially for the similar to 150 kDa catalytic subunit. Here we report the crystal structure of ChlH from the photosynthetic cyanobacterium Synechocystis PCC 6803, solved at 2.5 angstrom resolution. The active site is buried deeply inside the protein interior, and the surrounding residues are conserved throughout evolution. This structure helps to explain the loss of function reported for the cch and gun5 mutations of the ChlH subunit, and to provide the molecular basis of substrate channelling during the magnesium-chelating process. The structure advances our understanding of the holoenzyme of MgCh, a metal chelating enzyme other than ferrochelatase. |
学科领域 | Plant Sciences |
DOI | 10.1038/NPLANTS.2015.125 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | PROTOPORPHYRINOGEN-IX OXIDASE ; MG-CHELATASE ; H-SUBUNIT ; SYNECHOCYSTIS PCC6803 ; ATPASE ACTIVITY ; BARLEY MUTANTS ; ENZYME ; GUN4 ; BIOSYNTHESIS ; EXPRESSION |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000364383900002 |
出版者 | NATURE PUBLISHING GROUP |
文献子类 | Article |
出版地 | LONDON |
EISSN | 2055-0278 |
资助机构 | National Basic Research Program of China [2011CBA00901] ; Hundred Talents Program of Chinese Academy of Sciences ; [KGZD-EW-T05] |
作者邮箱 | wgong@ustc.edu.cn ; liulin@ibcas.ac.cn |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25941 |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Lab Noncoding RNA, Beijing 100101, Peoples R China 4.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China 5.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Xuemin,Pu, Hua,Fang, Ying,et al. Crystal structure of the catalytic subunit of magnesium chelatase[J]. NATURE PLANTS,2015,1(9). |
APA | Chen, Xuemin.,Pu, Hua.,Fang, Ying.,Wang, Xiao.,Zhao, Shun.,...&Liu, Lin.(2015).Crystal structure of the catalytic subunit of magnesium chelatase.NATURE PLANTS,1(9). |
MLA | Chen, Xuemin,et al."Crystal structure of the catalytic subunit of magnesium chelatase".NATURE PLANTS 1.9(2015). |
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Chen-2015-Crystal st(2618KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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