Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. 2KEN8 mature pollen

doi:10.3389/fpls.2015.00548

IB-CAS > 中科院光生物学重点实验室

	Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. 2KEN8 mature pollen
	Zhang, Jin ; Wu, Li-Shuan ; Fan, Wei ; Zhang, Xiao-Ling ; Jia, Hui-Xia ; Li, Yu ; Yin, Ya-Fang ; Hu, Jian-Jun ; Lu, Meng-Zhu
	2015
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	6
摘要	Proteomic analysis was used to generate a map of Populus deltoides CL. 2KEN8 mature pollen proteins. By applying 2-D electrophoresis, we resolved 403 protein spots from mature pollen. Using the matrix-assisted laser desorption/ionization time time-of-flight/time-of-flight tandem mass spectrometry method, we identified 178 distinct proteins from 218 protein spots expressed in mature pollen. Moreover, out of these, 28 proteins were identified as putative allergens. The expression patterns of these putative allergen genes indicate that several of these genes are highly expressed in pollen. In addition, the members of profilin allergen family were analyzed and their expression patterns were compared with their homologous genes in Arabidopsis and rice. Knowledge of these identified allergens has the potential to improve specific diagnosis and allergen immunotherapy treatment for patients with poplar pollen allergy.
关键词	allergen MALDI-TOF/TOF MS/MS pollen Populus deltoids profilin proteomics two-dimensional gel electrophoresis
学科领域	Plant Sciences
DOI	10.3389/fpls.2015.00548
收录类别	SCI
语种	英语
WOS关键词	CROSS-REACTIVITY ; 2-DIMENSIONAL ELECTROPHORESIS ; HEAT-STRESS ; ARABIDOPSIS ; EXPRESSION ; IDENTIFICATION ; PROFILIN ; REVEALS ; CLASSIFICATION ; AEROALLERGENS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000358726300002
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	Special Fund for Forestry Scientific Research in the Public Interest [201304103-4] ; National High-tech R&D Program of China [2011AA100201]
作者邮箱	Hujj@caf.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25942
专题	中科院光生物学重点实验室
作者单位	1.[Zhang, Jin 2.Wu, Li-Shuan 3.Fan, Wei 4.Zhang, Xiao-Ling 5.Jia, Hui-Xia 6.Li, Yu 7.Hu, Jian-Jun 8.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry A, Res Inst Forestry, Beijing 100091, Peoples R China 9.Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing, Jiangsu, Peoples R China 10.Chinese Acad Forestry, Res Inst Wood Ind, Beijing, Peoples R China 11.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 12.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jin,Wu, Li-Shuan,Fan, Wei,et al. Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. 2KEN8 mature pollen[J]. FRONTIERS IN PLANT SCIENCE,2015,6.
APA	Zhang, Jin.,Wu, Li-Shuan.,Fan, Wei.,Zhang, Xiao-Ling.,Jia, Hui-Xia.,...&Lu, Meng-Zhu.(2015).Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. 2KEN8 mature pollen.FRONTIERS IN PLANT SCIENCE,6.
MLA	Zhang, Jin,et al."Proteomic analysis and candidate allergenic proteins in Populus deltoides CL. 2KEN8 mature pollen".FRONTIERS IN PLANT SCIENCE 6(2015).

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