The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation

doi:10.1186/s12864-015-2047-6

	The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation
	Peng, Xianjun; Teng, Linhong; Yan, Xueqing; Zhao, Meiling; Shen, Shihua
	2015
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	16
摘要	Background: Most studies on the paper mulberry are mainly focused on the medicated and pharmacology, fiber quality, leaves feed development, little is known about its mechanism of adaptability to abiotic stress. Physiological measurement, transcriptomics and proteomic analysis were employed to understand its response to cold stress in this study. Methods: The second to fourth fully expanded leaves from up to down were harvested at different stress time points for the transmission electron microscope (TEM) observation. Physiological characteristics measurement included the relative electrolyte leakage (REL), SOD activity assay, soluble sugar content, and Chlorophyll fluorescence parameter measurement. For screening of differentially expressed genes, the expression level of every transcript in each sample was calculated by quantifying the number of Illumina reads. To identify the differentially expressed protein, leaves of plants under 0, 6, 12, 24, 48 and 72 h cold stress were harvested for proteomic analysis. Finally, real time PCR was used to verify the DEG results of the RNA-seq and the proteomics data. Results: Results showed that at the beginning of cold stress, respiratory metabolism was decreased and the transportation and hydrolysis of photosynthetic products was inhibited, leading to an accumulation of starch in the chloroplasts. Total of 5800 unigenes and 38 proteins were affected, including the repressed expression of photosynthesis and the enhanced expression in signal transduction, stress defense pathway as well as secondary metabolism. Although the transcriptional level of a large number of genes has been restored after 12 h, sustained cold stress brought more serious injury to the leaf cells, including the sharp rise of the relative electrolyte leakage, the declined Fv/Fm value, swelled chloroplast and the disintegrated membrane system. Conclusion: The starch accumulation and the photoinhibition might be the main adaptive mechanism of the paper mulberry responded to cold stress. Most of important, enhancing the transport and hydrolysis of photosynthetic products could be the potential targets for improving the cold tolerance of the paper mulberry.
关键词	Paper mulberry Transcriptomics Proteomics Abiotic stress Starch synthesis
学科领域	Biotechnology & Applied Microbiology ; Genetics & Heredity
DOI	10.1186/s12864-015-2047-6
收录类别	SCI
语种	英语
WOS关键词	PROTEOMIC ANALYSIS ; OXIDATIVE STRESS ; LOW-TEMPERATURE ; BROUSSONETIA-PAPYRIFERA ; ARABIDOPSIS-THALIANA ; FREEZING TOLERANCE ; CHILLING STRESS ; GENE-EXPRESSION ; ACCLIMATION ; TRANSCRIPTOME
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000365281100001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	Huimin Technology Demonstration Project of the National Modern Agricultural Science and Technology Achievements City [Z151100001015008] ; Knowledge Innovation Program through the Chinese Academy of Sciences [KZCX2-YW-359-2] ; National Natural Science Foundation of China [31270653]
作者邮箱	shshen@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：43[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25944
专题	中科院北方资源植物重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Peng, Xianjun,Teng, Linhong,Yan, Xueqing,et al. The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation[J]. BMC GENOMICS,2015,16.
APA	Peng, Xianjun,Teng, Linhong,Yan, Xueqing,Zhao, Meiling,&Shen, Shihua.(2015).The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation.BMC GENOMICS,16.
MLA	Peng, Xianjun,et al."The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation".BMC GENOMICS 16(2015).

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