IB-CAS  > 中科院北方资源植物重点实验室
The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation
Peng, Xianjun; Teng, Linhong; Yan, Xueqing; Zhao, Meiling; Shen, Shihua
Source PublicationBMC GENOMICS
AbstractBackground: Most studies on the paper mulberry are mainly focused on the medicated and pharmacology, fiber quality, leaves feed development, little is known about its mechanism of adaptability to abiotic stress. Physiological measurement, transcriptomics and proteomic analysis were employed to understand its response to cold stress in this study. Methods: The second to fourth fully expanded leaves from up to down were harvested at different stress time points for the transmission electron microscope (TEM) observation. Physiological characteristics measurement included the relative electrolyte leakage (REL), SOD activity assay, soluble sugar content, and Chlorophyll fluorescence parameter measurement. For screening of differentially expressed genes, the expression level of every transcript in each sample was calculated by quantifying the number of Illumina reads. To identify the differentially expressed protein, leaves of plants under 0, 6, 12, 24, 48 and 72 h cold stress were harvested for proteomic analysis. Finally, real time PCR was used to verify the DEG results of the RNA-seq and the proteomics data. Results: Results showed that at the beginning of cold stress, respiratory metabolism was decreased and the transportation and hydrolysis of photosynthetic products was inhibited, leading to an accumulation of starch in the chloroplasts. Total of 5800 unigenes and 38 proteins were affected, including the repressed expression of photosynthesis and the enhanced expression in signal transduction, stress defense pathway as well as secondary metabolism. Although the transcriptional level of a large number of genes has been restored after 12 h, sustained cold stress brought more serious injury to the leaf cells, including the sharp rise of the relative electrolyte leakage, the declined Fv/Fm value, swelled chloroplast and the disintegrated membrane system. Conclusion: The starch accumulation and the photoinhibition might be the main adaptive mechanism of the paper mulberry responded to cold stress. Most of important, enhancing the transport and hydrolysis of photosynthetic products could be the potential targets for improving the cold tolerance of the paper mulberry.
KeywordPaper mulberry Transcriptomics Proteomics Abiotic stress Starch synthesis
Subject AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
Indexed BySCI
WOS Research AreaScience Citation Index Expanded (SCI-EXPANDED)
WOS IDWOS:000365281100001
Publication PlaceLONDON
Funding OrganizationHuimin Technology Demonstration Project of the National Modern Agricultural Science and Technology Achievements City [Z151100001015008] ; Knowledge Innovation Program through the Chinese Academy of Sciences [KZCX2-YW-359-2] ; National Natural Science Foundation of China [31270653]
Corresponding Author Emailshshen@ibcas.ac.cn
OAgold, Green Published
Citation statistics
Cited Times:43[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
AffiliationChinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
Recommended Citation
GB/T 7714
Peng, Xianjun,Teng, Linhong,Yan, Xueqing,et al. The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation[J]. BMC GENOMICS,2015,16.
APA Peng, Xianjun,Teng, Linhong,Yan, Xueqing,Zhao, Meiling,&Shen, Shihua.(2015).The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation.BMC GENOMICS,16.
MLA Peng, Xianjun,et al."The cold responsive mechanism of the paper mulberry: decreased photosynthesis capacity and increased starch accumulation".BMC GENOMICS 16(2015).
Files in This Item:
File Name/Size DocType Version Access License
26537770.pdf(3890KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Peng, Xianjun]'s Articles
[Teng, Linhong]'s Articles
[Yan, Xueqing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Peng, Xianjun]'s Articles
[Teng, Linhong]'s Articles
[Yan, Xueqing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Peng, Xianjun]'s Articles
[Teng, Linhong]'s Articles
[Yan, Xueqing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 26537770.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.