Proteome Analysis of Poplar Seed Vigor

doi:10.1371/journal.pone.0132509

	Proteome Analysis of Poplar Seed Vigor
	Zhang, Hong ; Wang, Wei-Qing ; Liu, Shu-Jun ; Moller, Ian Max 1; Song, Song-Quan
	2015
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	10 期号:7
摘要	Seed vigor is a complex property that determines the seed's potential for rapid uniform emergence and subsequent growth. However, the mechanism for change in seed vigor is poorly understood. The seeds of poplar (Populus x Canadensis Moench), which are short-lived, were stored at 30 degrees C and 75 +/- 5% relative humidity for different periods of time (0-90 days) to obtain different vigor seeds (from 95 to 0% germination). With decreasing seed vigor, the temperature range of seed germination became narrower; the respiration rate of the seeds decreased markedly, while the relative electrolyte leakage increased markedly, both levelling off after 45 days. A total of 81 protein spots showed a significant change in abundance (>= 1.5-fold, P < 0.05) when comparing the proteomes among seeds with different vigor. Of the identified 65 proteins, most belonged to the groups involved in metabolism (23%), protein synthesis and destination (22%), energy (18%), cell defense and rescue (17%), and storage protein (15%). These proteins accounted for 95% of all the identified proteins. During seed aging, 53 and 6 identified proteins consistently increased and decreased in abundance, respectively, and they were associated with metabolism (22%), protein synthesis and destination (22%), energy (19%), cell defense and rescue (19%), storage proteins (15%), and cell growth and structure (3%). These data show that the decrease in seed vigor (aging) is an energy-dependent process, which requires protein synthesis and degradation as well as cellular defense and rescue.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0132509
收录类别	SCI
语种	英语
WOS关键词	HEAT-SHOCK PROTEINS ; RIPARIAN COTTONWOODS ; EXPRESSION ANALYSIS ; INSTREAM FLOWS ; ACTIVE OXYGEN ; GENE FAMILY ; ARABIDOPSIS ; GERMINATION ; DETERIORATION ; GLUTATHIONE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000358194900055
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Science Foundation of China [31300529, 31171624] ; National Science and Technology Support Program [2012BAC01B05]
作者邮箱	sqsong@ibcas.ac.cn
作品OA属性	Green Published, gold, Green Submitted
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25948
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Aarhus Univ, Dept Mol Biol & Genet, Slagelse, Denmark
推荐引用方式 GB/T 7714	Zhang, Hong,Wang, Wei-Qing,Liu, Shu-Jun,et al. Proteome Analysis of Poplar Seed Vigor[J]. PLOS ONE,2015,10(7).
APA	Zhang, Hong,Wang, Wei-Qing,Liu, Shu-Jun,Moller, Ian Max,&Song, Song-Quan.(2015).Proteome Analysis of Poplar Seed Vigor.PLOS ONE,10(7).
MLA	Zhang, Hong,et al."Proteome Analysis of Poplar Seed Vigor".PLOS ONE 10.7(2015).

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