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Proteomic Analysis of Lettuce Seed Germination and Thermoinhibition by Sampling of Individual Seeds at Germination and Removal of Storage Proteins by Polyethylene Glycol Fractionation | |
Wang, Wei-Qing; Song, Bin-Yan; Deng, Zhi-Jun; Wang, Yue; Liu, Shu-Jun; Moller, Ian Max4; Song, Song-Quan | |
2015 | |
发表期刊 | PLANT PHYSIOLOGY |
ISSN | 0032-0889 |
卷号 | 167期号:4 |
摘要 | Germination and thermoinhibition in lettuce (Lactuca sativa 'Jianyexianfeng No. 1') seeds were investigated by a proteomic comparison among dry seeds, germinated seeds at 15 degrees C, at 15 degrees C after imbibition at 25 degrees C for 48 h, or at 25 degrees C in KNO3 (all sampled individually at germination), and ungerminated seeds at 25 degrees C, a thermoinhibitory temperature. Before two-dimensional gel electrophoresis analysis, storage proteins (greater than 50% of total extractable protein) were removed by polyethylene glycol precipitation, which significantly improved the detection of less abundant proteins on two-dimensional gels. A total of 108 protein spots were identified to change more than 2-fold (P < 0.05) in abundance in at least one germination treatment. Nineteen proteins increasing and one protein decreasing in abundance during germination had higher abundance in germinated 15 degrees C, 15 degrees C after imbibition at 25 degrees C for 48 h, and 25 degrees C in KNO3 seeds than in ungerminated 25 degrees C seeds. Gene expression of 12 of those proteins correlated well with the protein accumulation. Methionine metabolism, ethylene production, lipid mobilization, cell elongation, and detoxification of aldehydes were revealed to be potentially related to lettuce seed germination and thermoinhibition. Accumulation of three proteins and expression of five genes participating in the mevalonate (MVA) pathway of isoprenoid biosynthesis correlated positively with seed germinability. Inhibition of this pathway by lovastatin delayed seed germination and increased the sensitivity of germination to abscisic acid. MVA pathway-derived products, cytokinins, partially reversed the lovastatin inhibition of germination and released seed thermoinhibition at 25 degrees C. We conclude that the MVA pathway for isoprenoid biosynthesis is involved in lettuce seed germination and thermoinhibition. |
学科领域 | Plant Sciences |
DOI | 10.1104/pp.15.00045 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | ABSCISIC-ACID BIOSYNTHESIS ; ENDO-BETA-MANNANASE ; CV GRAND-RAPIDS ; HIGH-TEMPERATURE ; ARABIDOPSIS-THALIANA ; ISOPRENOID BIOSYNTHESIS ; SIGNAL-TRANSDUCTION ; STRUCTURAL PROTEINS ; ETHYLENE PRODUCTION ; ACTIN CYTOSKELETON |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000354438500012 |
出版者 | AMER SOC PLANT BIOLOGISTS |
文献子类 | Article |
出版地 | ROCKVILLE |
EISSN | 1532-2548 |
资助机构 | National Sciences and Technology Support Program [2012BAC01B05] ; National Natural Sciences Foundation of China [31171624, 81102757] ; Chinese Academy of Sciences |
作者邮箱 | sqsong@ibcas.ac.cn |
作品OA属性 | Bronze, Green Published |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/25950 |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 3.Jishou Univ, Coll Biol Resources & Environm Sci, Jishou 416000, Peoples R China 4.Hubei Minzu Univ, Coll Forestry & Hort, Enshi 445000, Peoples R China 5.Aarhus Univ, Dept Mol Biol & Genet, DK-4200 Slagelse, Denmark |
推荐引用方式 GB/T 7714 | Wang, Wei-Qing,Song, Bin-Yan,Deng, Zhi-Jun,et al. Proteomic Analysis of Lettuce Seed Germination and Thermoinhibition by Sampling of Individual Seeds at Germination and Removal of Storage Proteins by Polyethylene Glycol Fractionation[J]. PLANT PHYSIOLOGY,2015,167(4). |
APA | Wang, Wei-Qing.,Song, Bin-Yan.,Deng, Zhi-Jun.,Wang, Yue.,Liu, Shu-Jun.,...&Song, Song-Quan.(2015).Proteomic Analysis of Lettuce Seed Germination and Thermoinhibition by Sampling of Individual Seeds at Germination and Removal of Storage Proteins by Polyethylene Glycol Fractionation.PLANT PHYSIOLOGY,167(4). |
MLA | Wang, Wei-Qing,et al."Proteomic Analysis of Lettuce Seed Germination and Thermoinhibition by Sampling of Individual Seeds at Germination and Removal of Storage Proteins by Polyethylene Glycol Fractionation".PLANT PHYSIOLOGY 167.4(2015). |
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