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Proteomic Analysis of Lettuce Seed Germination and Thermoinhibition by Sampling of Individual Seeds at Germination and Removal of Storage Proteins by Polyethylene Glycol Fractionation
Wang, Wei-Qing; Song, Bin-Yan; Deng, Zhi-Jun; Wang, Yue; Liu, Shu-Jun; Moller, Ian Max4; Song, Song-Quan
2015
发表期刊PLANT PHYSIOLOGY
ISSN0032-0889
卷号167期号:4
摘要Germination and thermoinhibition in lettuce (Lactuca sativa 'Jianyexianfeng No. 1') seeds were investigated by a proteomic comparison among dry seeds, germinated seeds at 15 degrees C, at 15 degrees C after imbibition at 25 degrees C for 48 h, or at 25 degrees C in KNO3 (all sampled individually at germination), and ungerminated seeds at 25 degrees C, a thermoinhibitory temperature. Before two-dimensional gel electrophoresis analysis, storage proteins (greater than 50% of total extractable protein) were removed by polyethylene glycol precipitation, which significantly improved the detection of less abundant proteins on two-dimensional gels. A total of 108 protein spots were identified to change more than 2-fold (P < 0.05) in abundance in at least one germination treatment. Nineteen proteins increasing and one protein decreasing in abundance during germination had higher abundance in germinated 15 degrees C, 15 degrees C after imbibition at 25 degrees C for 48 h, and 25 degrees C in KNO3 seeds than in ungerminated 25 degrees C seeds. Gene expression of 12 of those proteins correlated well with the protein accumulation. Methionine metabolism, ethylene production, lipid mobilization, cell elongation, and detoxification of aldehydes were revealed to be potentially related to lettuce seed germination and thermoinhibition. Accumulation of three proteins and expression of five genes participating in the mevalonate (MVA) pathway of isoprenoid biosynthesis correlated positively with seed germinability. Inhibition of this pathway by lovastatin delayed seed germination and increased the sensitivity of germination to abscisic acid. MVA pathway-derived products, cytokinins, partially reversed the lovastatin inhibition of germination and released seed thermoinhibition at 25 degrees C. We conclude that the MVA pathway for isoprenoid biosynthesis is involved in lettuce seed germination and thermoinhibition.
学科领域Plant Sciences
DOI10.1104/pp.15.00045
收录类别SCI
语种英语
WOS关键词ABSCISIC-ACID BIOSYNTHESIS ; ENDO-BETA-MANNANASE ; CV GRAND-RAPIDS ; HIGH-TEMPERATURE ; ARABIDOPSIS-THALIANA ; ISOPRENOID BIOSYNTHESIS ; SIGNAL-TRANSDUCTION ; STRUCTURAL PROTEINS ; ETHYLENE PRODUCTION ; ACTIN CYTOSKELETON
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000354438500012
出版者AMER SOC PLANT BIOLOGISTS
文献子类Article
出版地ROCKVILLE
EISSN1532-2548
资助机构National Sciences and Technology Support Program [2012BAC01B05] ; National Natural Sciences Foundation of China [31171624, 81102757] ; Chinese Academy of Sciences
作者邮箱sqsong@ibcas.ac.cn
作品OA属性Bronze, Green Published
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/25950
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China
3.Jishou Univ, Coll Biol Resources & Environm Sci, Jishou 416000, Peoples R China
4.Hubei Minzu Univ, Coll Forestry & Hort, Enshi 445000, Peoples R China
5.Aarhus Univ, Dept Mol Biol & Genet, DK-4200 Slagelse, Denmark
推荐引用方式
GB/T 7714
Wang, Wei-Qing,Song, Bin-Yan,Deng, Zhi-Jun,et al. Proteomic Analysis of Lettuce Seed Germination and Thermoinhibition by Sampling of Individual Seeds at Germination and Removal of Storage Proteins by Polyethylene Glycol Fractionation[J]. PLANT PHYSIOLOGY,2015,167(4).
APA Wang, Wei-Qing.,Song, Bin-Yan.,Deng, Zhi-Jun.,Wang, Yue.,Liu, Shu-Jun.,...&Song, Song-Quan.(2015).Proteomic Analysis of Lettuce Seed Germination and Thermoinhibition by Sampling of Individual Seeds at Germination and Removal of Storage Proteins by Polyethylene Glycol Fractionation.PLANT PHYSIOLOGY,167(4).
MLA Wang, Wei-Qing,et al."Proteomic Analysis of Lettuce Seed Germination and Thermoinhibition by Sampling of Individual Seeds at Germination and Removal of Storage Proteins by Polyethylene Glycol Fractionation".PLANT PHYSIOLOGY 167.4(2015).
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