Fatty acid composition of developing tree peony (Paeonia section Moutan DC.) seeds and transcriptome analysis during seed development

doi:10.1186/s12864-015-1429-0

	Fatty acid composition of developing tree peony (Paeonia section Moutan DC.) seeds and transcriptome analysis during seed development
	Li, Shan-Shan ; Wang, Liang-Sheng; Shu, Qing-Yan ; Wu, Jie ; Chen, Li-Guang ; Shao, Shuai 4; Yin, Dan-Dan
	2015
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	16
摘要	Background: Tree peony (Paeonia section Moutan DC.) is known for its excellent ornamental and medicinal values. In 2011, seeds from P. ostii have been identified as novel resource of a-linolenic acid (ALA) for seed oil production and development in China. However, the molecular mechanism on biosynthesis of unsaturated fatty acids in tree peony seeds remains unknown. Therefore, transcriptome data is needed to better understand the underlying mechanisms. Results: In this study, lipid accumulation contents were measured using GC-MS methods across developing tree peony seeds, which exhibited an extraordinary ALA content (49.3%) in P. ostii mature seeds. Transcriptome analysis was performed using Illumina sequencing platform. A total of 144 million 100-bp paired-end reads were generated from six libraries, which identified 175,874 contigs. In the KEGG Orthology enrichment of differentially expressed genes, lipid metabolism pathways were highly represented categories. Using this data we identified 388 unigenes that may be involved in de novo fatty acid and triacylglycerol biosynthesis. In particular, three unigenes (SAD, FAD2 and FAD8) encoding fatty acid desaturase with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified. Conclusions: This study provides the first comprehensive genomic resources characterizing tree peony seeds gene expression at the transcriptional level. These data lay the foundation for further understanding of molecular mechanism responsible for lipid biosynthesis and the high unsaturated fatty acids (especially ALA) accumulation. Meanwhile, it provides theoretical base for potential oilseed application in the respect of n-6 to n-3 ratio for human diets and future regulation of target healthy components of oils.
关键词	Paeoniaceae Tree peony Transcriptome alpha-linolenic acid Omega-3 fatty acid Triacylglycerols
学科领域	Biotechnology & Applied Microbiology ; Genetics & Heredity
DOI	10.1186/s12864-015-1429-0
收录类别	SCI
语种	英语
WOS关键词	FUNCTIONAL ANNOTATION ; GAS-CHROMATOGRAPHY ; OIL BODIES ; GENE ; HEALTH ; IDENTIFICATION ; BIOSYNTHESIS ; CULTIVARS ; OLEOSINS ; DISEASE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000353011600001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National High Technology Research and Development Program of China (863 Program) [2011AA10020702]
作者邮箱	wanglsh@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：77[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25951
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Forestry, Inst Forest Protect, Beijing 100091, Peoples R China 5.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Li, Shan-Shan,Wang, Liang-Sheng,Shu, Qing-Yan,et al. Fatty acid composition of developing tree peony (Paeonia section Moutan DC.) seeds and transcriptome analysis during seed development[J]. BMC GENOMICS,2015,16.
APA	Li, Shan-Shan.,Wang, Liang-Sheng.,Shu, Qing-Yan.,Wu, Jie.,Chen, Li-Guang.,...&Yin, Dan-Dan.(2015).Fatty acid composition of developing tree peony (Paeonia section Moutan DC.) seeds and transcriptome analysis during seed development.BMC GENOMICS,16.
MLA	Li, Shan-Shan,et al."Fatty acid composition of developing tree peony (Paeonia section Moutan DC.) seeds and transcriptome analysis during seed development".BMC GENOMICS 16(2015).

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