A novel system for evaluating drought-cold tolerance of grapevines using chlorophyll fluorescence

doi:10.1186/s12870-015-0459-8

	A novel system for evaluating drought-cold tolerance of grapevines using chlorophyll fluorescence
	Su, Lingye 1,2; Dai, Zhanwu3 ; Li, Shaohua1,4 ; Xin, Haiping 4
	2015
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	15
摘要	Background: Grape production in continental climatic regions suffers from the combination of drought and cold stresses during winter. Developing a reliable system to simulate combined drought-cold stress and to determine physiological responses and regulatory mechanisms is important. Evaluating tolerance to combined stress at germplasm level is crucial to select parents for breeding grapevines. Results: In the present study, two species, namely, Vitis amurensis and V. vinifera cv. 'Muscat Hamburg', were used to develop a reliable system for evaluating their tolerance to drought-cold stress. This system used tissue-cultured grapevine plants, 6% PEG solution, and gradient cooling mode to simulate drought-cold stress. V. amurensis had a significantly lower LT50 value (the temperature of 50% electrolyte leakage) than 'Muscat Hamburg' during simulated drought-cold stress. Thus, the former had higher tolerance than the latter to drought-cold stress based on electrolyte leakage (EL) measurements. Moreover, the chlorophyll fluorescence responses of V. amurensis and 'Muscat Hamburg' were also analyzed under drought-cold stress. The maximum photochemical quantum yield of PS II (Fv/Fm) exhibited a significant linear correlationship with EL. The relationship of EL with Fv/Fm in the other four genotypes of grapevines under drought-cold stress was also detected. Conclusions: A novel LT50 estimation model was established, and the LT50 values can be well calculated based on Fv/Fm in replacement of EL measurement. The Fv/Fm-based model exhibits good reliability for evaluating the tolerance of different grapevine genotypes to drought-cold stress.
关键词	Drought-cold stress Electrolyte leakage Fv/Fm Grapevine LT50
学科领域	Plant Sciences
DOI	10.1186/s12870-015-0459-8
收录类别	SCI
语种	英语
WOS关键词	EXPRESSION ANALYSIS ; FREEZING TOLERANCE ; STRESS TOLERANCE ; RESPONSES ; VITIS ; HEAT ; ARABIDOPSIS ; ACCLIMATION ; COMBINATION ; RESISTANCE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000351202600001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of China (NSFC Accession) [31130047, 31471857] ; Youth Innovation Promotion Association of CAS [2015281]
作者邮箱	shhli@ibcas.ac.cn ; xinhaiping215@hotmail.com
作品OA属性	Green Published, gold
引用统计	被引频次：166[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25952
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.INRA, Inst Sci Vigne & Vin, UMR EGFV 1287, F-33882 Villenave DOrnon, France 5.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty, Wuhan Bot Garden, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Su, Lingye,Dai, Zhanwu,Li, Shaohua,et al. A novel system for evaluating drought-cold tolerance of grapevines using chlorophyll fluorescence[J]. BMC PLANT BIOLOGY,2015,15.
APA	Su, Lingye,Dai, Zhanwu,Li, Shaohua,&Xin, Haiping.(2015).A novel system for evaluating drought-cold tolerance of grapevines using chlorophyll fluorescence.BMC PLANT BIOLOGY,15.
MLA	Su, Lingye,et al."A novel system for evaluating drought-cold tolerance of grapevines using chlorophyll fluorescence".BMC PLANT BIOLOGY 15(2015).

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