Construction of a high-density genetic map and QTLs mapping for sugars and acids in grape berries

doi:10.1186/s12870-015-0428-2

	Construction of a high-density genetic map and QTLs mapping for sugars and acids in grape berries
	Chen, Jie 2; Wang, Nian ; Fang, Lin-Chuan ; Liang, Zhen-Chang ; Li, Shao-Hua; Wu, Ben-Hong
	2015
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	15
摘要	Background: QTLs controlling individual sugars and acids (fructose, glucose, malic acid and tartaric acid) in grape berries have not yet been identified. The present study aimed to construct a high-density, high-quality genetic map of a winemaking grape cross with a complex parentage (V. vinifera x V. amurensis) x ((V. labrusca x V. riparia) x V. vinifera), using next-generation restriction site-associated DNA sequencing, and then to identify loci related to phenotypic variability over three years. Results: In total, 1 826 SNP-based markers were developed. Of these, 621 markers were assembled into 19 linkage groups (LGs) for the maternal map, 696 for the paternal map, and 1 254 for the integrated map. Markers showed good linear agreement on most chromosomes between our genetic maps and the previously published V. vinifera reference sequence. However marker order was different in some chromosome regions, indicating both conservation and variation within the genome. Despite the identification of a range of QTLs controlling the traits of interest, these QTLs explained a relatively small percentage of the observed phenotypic variance. Although they exhibited a large degree of instability from year to year, QTLs were identified for all traits but tartaric acid and titratable acidity in the three years of the study; however only the QTLs for malic acid and beta ratio (tartaric acid-to-malic acid ratio) were stable in two years. QTLs related to sugars were located within ten LGs (01, 02, 03, 04, 07, 09, 11, 14, 17, 18), and those related to acids within three LGs (06, 13, 18). Overlapping QTLs in LG14 were observed for fructose, glucose and total sugar. Malic acid, total acid and beta ratio each had several QTLs in LG18, and malic acid also had a QTL in LG06. A set of 10 genes underlying these QTLs may be involved in determining the malic acid content of berries. Conclusion: The genetic map constructed in this study is potentially a high-density, high-quality map, which could be used for QTL detection, genome comparison, and sequence assembly. It may also serve to broaden our understanding of the grape genome.
关键词	Berry quality Genetic map Next-generation sequencing (NGS) QTL analysis Quantitative trait loci Restriction-site associated DNA (RAD) Vitis
学科领域	Plant Sciences
DOI	10.1186/s12870-015-0428-2
收录类别	SCI
语种	英语
WOS关键词	VITIS-VINIFERA L. ; POWDERY MILDEW RESISTANCE ; LINKAGE MAP ; PIERCES-DISEASE ; FRUIT-QUALITY ; ADVANCED BACKCROSS ; ACTIVATED CITRATE ; DE-NOVO ; TRAITS ; IDENTIFICATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000349409900003
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of China [NSFC 31372030, NSFC 31130047]
作者邮箱	shhli@ibcas.ac.cn ; bhwu@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：71[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25953
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jie,Wang, Nian,Fang, Lin-Chuan,et al. Construction of a high-density genetic map and QTLs mapping for sugars and acids in grape berries[J]. BMC PLANT BIOLOGY,2015,15.
APA	Chen, Jie,Wang, Nian,Fang, Lin-Chuan,Liang, Zhen-Chang,Li, Shao-Hua,&Wu, Ben-Hong.(2015).Construction of a high-density genetic map and QTLs mapping for sugars and acids in grape berries.BMC PLANT BIOLOGY,15.
MLA	Chen, Jie,et al."Construction of a high-density genetic map and QTLs mapping for sugars and acids in grape berries".BMC PLANT BIOLOGY 15(2015).

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