A proteomic analysis of seeds from Bt-transgenic Brassica napus and hybrids with wild B. juncea

doi:10.1038/srep15480

	A proteomic analysis of seeds from Bt-transgenic Brassica napus and hybrids with wild B. juncea
	Liu, Yongbo ; Zhang, Ying-Xue ; Song, Song-Quan ; Li, Junsheng ; Stewart, C. Neal, Jr.; Wei, Wei 3; Zhao, Yujie ; Wang, Wei-Qing
	2015
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	5
摘要	Transgene insertions might have unintended side effects on the transgenic host, both crop and hybrids with wild relatives that harbor transgenes. We employed proteomic approaches to assess protein abundance changes in seeds from Bt-transgenic oilseed rape (Brassica napus) and its hybrids with wild mustard (B. juncea). A total of 24, 15 and 34 protein spots matching to 23, 13 and 31 unique genes were identified that changed at least 1.5 fold (p < 0.05, Student's t-test) in abundance between transgenic (tBN) and non-transgenic (BN) oilseed rape, between hybrids of B. juncea (BJ) x tBN (BJtBN) and BJ x BN (BJBN) and between BJBN and BJ, respectively. Eight proteins had higher abundance in tBN than in BN. None of these proteins was toxic or nutritionally harmful to human health, which is not surprising since the seeds are not known to produce toxic proteins. Protein spots varying in abundance between BJtBN and BJBN seeds were the same or homologous to those in the respective parents. None of the differentially-accumulated proteins between BJtBN and BJBN were identical to those between tBN and BN. Results indicated that unintended effects resulted from transgene flow fell within the range of natural variability of hybridization and those found in the native host proteomes.
学科领域	Multidisciplinary Sciences
DOI	10.1038/srep15480
收录类别	SCI
语种	英语
WOS关键词	MEDICAGO-TRUNCATULA SEEDS ; HEAT-STABLE PROTEOME ; SUBSTANTIAL EQUIVALENCE ; UNINTENDED CHANGES ; CROP ALLELES ; PROTEINS ; GENE ; INTROGRESSION ; EXPRESSION ; PLANTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000363123000001
出版者	NATURE RESEARCH
文献子类	Article
出版地	BERLIN
资助机构	Natural Science Foundation of China [31200288] ; National Environmental Protection Public Welfare Science and Technology Research Program of China [201309038] ; one Project of State Key Laboratory of Environmental Criteria and Risk Assessment [SKLECRA2013OFP14]
作者邮箱	liuyb@craes.org.cn ; wwq0814@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/25965
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Beijing Bot Garden, Inst Bot, Beijing 100093, Peoples R China 4.Chinese Acad Sci, State Key Lab Vegetat & Environm Change, Inst Bot, Beijing 100093, Peoples R China 5.Henan Univ, Coll Chem & Chem Engn, Kaifeng 475001, Peoples R China 6.Stewart, C. Neal, Jr.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA
推荐引用方式 GB/T 7714	Liu, Yongbo,Zhang, Ying-Xue,Song, Song-Quan,et al. A proteomic analysis of seeds from Bt-transgenic Brassica napus and hybrids with wild B. juncea[J]. SCIENTIFIC REPORTS,2015,5.
APA	Liu, Yongbo.,Zhang, Ying-Xue.,Song, Song-Quan.,Li, Junsheng.,Stewart, C. Neal, Jr..,...&Wang, Wei-Qing.(2015).A proteomic analysis of seeds from Bt-transgenic Brassica napus and hybrids with wild B. juncea.SCIENTIFIC REPORTS,5.
MLA	Liu, Yongbo,et al."A proteomic analysis of seeds from Bt-transgenic Brassica napus and hybrids with wild B. juncea".SCIENTIFIC REPORTS 5(2015).

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