Evaluation of Four Commonly Used DNA Barcoding Loci for Chinese Medicinal Plants of the Family Schisandraceae

doi:10.1371/journal.pone.0125574

	Evaluation of Four Commonly Used DNA Barcoding Loci for Chinese Medicinal Plants of the Family Schisandraceae
	Zhang, Jian ; Chen, Min 1; Dong, Xiaoyu 2; Lin, Ruozhu 3; Fan, Jianhua ; Chen, Zhiduan
	2015
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	10 期号:5
摘要	Many species of Schisandraceae are used in traditional Chinese medicine and are faced with contamination and substitution risks due to inaccurate identification. Here, we investigated the discriminatory power of four commonly used DNA barcoding loci (ITS, trnH-psbA, matK, and rbcL) and corresponding multi-locus combinations for 135 individuals from 33 species of Schisandraceae, using distance-, tree-, similarity-, and character-based methods, at both the family level and the genus level. Our results showed that the two spacer regions (ITS and trnH-psbA) possess higher species-resolving power than the two coding regions (matK and rbcL). The degree of species resolution increased with most of the multilocus combinations. Furthermore, our results implied that the best DNA barcode for the species discrimination at the family level might not always be the most suitable one at the genus level. Here we propose the combination of ITS+trnH-psbA+matK+rbcL as the most ideal DNA barcode for discriminating the medicinal plants of Schisandra and Kadsura, and the combination of ITS+trnH-psbA as the most suitable barcode for Illicium species. In addition, the closely related species Schisandra rubriflora Rehder & E. H. Wilson and Schisandra grandifloraHook.f. & Thomson, were paraphyletic with each other on phylogenetic trees, suggesting that they should not be distinct species. Furthermore, the samples of these two species from the southern Hengduan Mountains region formed a distinct cluster that was separated from the samples of other regions, implying the presence of cryptic diversity. The feasibility of DNA barcodes for identification of geographical authenticity was also verified here. The database and paradigm that we provide in this study could be used as reference for the authentication of traditional Chinese medicinal plants utilizing DNA barcoding.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0125574
收录类别	SCI
语种	英语
WOS关键词	MOLECULAR PHYLOGENETICS ; CHEMICAL-CONSTITUENTS ; COMMUNITY PHYLOGENY ; MAXIMUM-LIKELIHOOD ; TRNH-PSBA ; IDENTIFICATION ; RBCL ; BIODIVERSITY ; SEQUENCES ; CHINENSIS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000353943000077
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Natural Science Foundation of China [31100171, 31270268] ; Major Innovation Program of Chinese Academy of Sciences [KSCX2-EW-Z-2] ; National Basic Research Program of China [2014CB954101] ; CAS International Research & Education Development Program [SAJC201315] ; CAS External Cooperation Program of BIC [GJ II Z201321]
作者邮箱	zhiduan@ibcas.ac.cn
作品OA属性	Green Published, gold, Green Submitted
引用统计	被引频次：28[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26015
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Jiangsu Prov & Chinese Acad Sci, Nanjing Bot Garden Mem Sun Yat Sen, Inst Bot, Nanjing, Jiangsu, Peoples R China 4.Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jian,Chen, Min,Dong, Xiaoyu,et al. Evaluation of Four Commonly Used DNA Barcoding Loci for Chinese Medicinal Plants of the Family Schisandraceae[J]. PLOS ONE,2015,10(5).
APA	Zhang, Jian,Chen, Min,Dong, Xiaoyu,Lin, Ruozhu,Fan, Jianhua,&Chen, Zhiduan.(2015).Evaluation of Four Commonly Used DNA Barcoding Loci for Chinese Medicinal Plants of the Family Schisandraceae.PLOS ONE,10(5).
MLA	Zhang, Jian,et al."Evaluation of Four Commonly Used DNA Barcoding Loci for Chinese Medicinal Plants of the Family Schisandraceae".PLOS ONE 10.5(2015).

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