Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia

doi:10.1371/journal.pone.0115168

	Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia
	Xu, Songzhi ; Li, Dezhu 1; Li, Jianwu 2; Xiang, Xiaoguo ; Jin, Weitao ; Huang, Weichang 3; Jin, Xiaohua; Huang, Luqi 4
	2015
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	10 期号:1
摘要	DNA barcoding has been proposed to be one of the most promising tools for accurate and rapid identification of taxa. However, few publications have evaluated the efficiency of DNA barcoding for the large genera of flowering plants. Dendrobium, one of the largest genera of flowering plants, contains many species that are important in horticulture, medicine and biodiversity conservation. Besides, Dendrobium is a notoriously difficult group to identify. DNA barcoding was expected to be a supplementary means for species identification, conservation and future studies in Dendrobium. We assessed the power of 11 candidate barcodes on the basis of 1,698 accessions of 184 Dendrobium species obtained primarily from mainland Asia. Our results indicated that five single barcodes, i.e., ITS, ITS2, matK, rbcL and trnH-psbA, can be easily amplified and sequenced with the currently established primers. Four barcodes, ITS, ITS2, ITS+matK, and ITS2+matK, have distinct barcoding gaps. ITS+matK was the optimal barcode based on all evaluation methods. Furthermore, the efficiency of ITS+matK was verified in four other large genera including Ficus, Lysimachia, Paphiopedilum, and Pedicularis in this study. Therefore, we tentatively recommend the combination of ITS+ matK as a core DNA barcode for large flowering plant genera.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0115168
收录类别	SCI
语种	英语
WOS关键词	INTERNAL TRANSCRIBED SPACER ; PHYLOGENETIC ANALYSIS ; SYSTEMATICS ; IDENTIFICATION ; DISCRIMINATE ; SEQUENCES ; TAXONOMY ; RATES ; MATK ; LOCI
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000348203500009
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Natural Science Foundation of China [31107176, 31470299, J1310002] ; Chinese Special Fund for Medicine Research in the Public Interest [201407003]
作者邮箱	xiaohuajin@ibcas.ac.cn ; huangluqi01@126.com
作品OA属性	Green Submitted, Green Published, gold
引用统计	被引频次：62[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26023
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Div & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China 3.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Menglun Township 666303, Yunnan, Peoples R China 4.Shanghai Chenshan Bot Garden, Shanghai 201602, Peoples R China 5.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China
推荐引用方式 GB/T 7714	Xu, Songzhi,Li, Dezhu,Li, Jianwu,et al. Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia[J]. PLOS ONE,2015,10(1).
APA	Xu, Songzhi.,Li, Dezhu.,Li, Jianwu.,Xiang, Xiaoguo.,Jin, Weitao.,...&Huang, Luqi.(2015).Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia.PLOS ONE,10(1).
MLA	Xu, Songzhi,et al."Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia".PLOS ONE 10.1(2015).

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