Cytological and proteomic analyses of horsetail (Equisetum arvense L.) spore germination

doi:10.3389/fpls.2015.00441

	Cytological and proteomic analyses of horsetail (Equisetum arvense L.) spore germination
	Zhao, Qi ; Gao, Jing 1; Suo, Jinwei 1; Chen, Sixue 2; Wang, Tai3 ; Dai, Shaojun
	2015
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	6
摘要	Spermatophyte pollen tubes and root hairs have been used as single-cell-type model systems to understand the molecular processes underlying polar growth of plant cells. Horsetail (Equisetum arvense L.) is a perennial herb species in Equisetopsida, which creates separately growing spring and summer stems in its life cycle. The mature chlorophyllous spores produced from spring stems can germinate without dormancy. Here we report the cellular features and protein expression patterns in five stages of horsetail spore germination (mature spores, rehydrated spores, double-celled spores, germinated spores, and spores with protonemal cells). Using 2-DE combined with mass spectrometry, 80 proteins were found to be abundance changed upon spore germination. Among them, proteins involved in photosynthesis, protein turnover, and energy supply were over-represented. Thirteen proteins appeared as proteoforms on the gels, indicating the potential importance of post-translational modification. In addition, the dynamic changes of ascorbate peroxidase, peroxiredoxin, and dehydroascorbate reductase implied that reactive oxygen species homeostasis is critical in regulating cell division and tip-growth. The time course of germination and diverse expression patterns of proteins in photosynthesis, energy supply, lipid and amino acid metabolism indicated that heterotrophic and autotrophic metabolism were necessary in light-dependent germination of the spores. Twenty-six proteins were involved in protein synthesis, folding, and degradation, indicating that protein turnover is vital to spore germination and rhizoid tip-growth. Furthermore, the altered abundance of 14-3-3 protein, small G protein Ran, actin, and caffeoyl-CoA O-methyltransferase revealed that signaling transduction, vesicle trafficking, cytoskeleton dynamics, and cell wall modulation were critical to cell division and polar growth. These findings lay a foundation toward understanding the molecular mechanisms underlying fern spore asymmetric division and rhizoid polar growth.
关键词	spore germination Equisetum arvense L. fern proteomics single cell polar growth
学科领域	Plant Sciences
DOI	10.3389/fpls.2015.00441
收录类别	SCI
语种	英语
WOS关键词	POLARIZED CELL-GROWTH ; POLLEN GERMINATION ; SIGNALING PATHWAYS ; ONOCLEA-SENSIBILIS ; CALCIUM CURRENT ; REDUCTASE GENE ; NITRIC-OXIDE ; FERN ; EXPRESSION ; PROTEINS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000357289100001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	National Natural Science Foundation of China [31071194] ; Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning ; Capacity Construction Project of Local Universities, Shanghai, China [14390502700] ; Funding Program for Young Teachers at Universities and Colleges of Shanghai ; Shanghai Normal University [SK201419]
作者邮箱	daishaojun@hotmail.com
作品OA属性	Green Published, gold
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26030
专题	中科院植物分子生理学重点实验室
作者单位	1.Shanghai Normal Univ, Coll Life & Environm Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China 2.Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Minist Educ, Key Lab Saline Alkali Vegetat Ecol Restorat Oil F, Harbin, Peoples R China 3.Univ Florida, Genet Inst, Interdisciplinary Ctr Biotechnol Res, Dept Biol,Plant Mol & Cellular Biol Program, Gainesville, FL USA 4.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Qi,Gao, Jing,Suo, Jinwei,et al. Cytological and proteomic analyses of horsetail (Equisetum arvense L.) spore germination[J]. FRONTIERS IN PLANT SCIENCE,2015,6.
APA	Zhao, Qi,Gao, Jing,Suo, Jinwei,Chen, Sixue,Wang, Tai,&Dai, Shaojun.(2015).Cytological and proteomic analyses of horsetail (Equisetum arvense L.) spore germination.FRONTIERS IN PLANT SCIENCE,6.
MLA	Zhao, Qi,et al."Cytological and proteomic analyses of horsetail (Equisetum arvense L.) spore germination".FRONTIERS IN PLANT SCIENCE 6(2015).

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