Multiple and integrated functions of floral C-class MADS-box genes in flower and fruit development of Physalis floridana

doi:10.1007/s11103-021-01182-4

	Multiple and integrated functions of floral C-class MADS-box genes in flower and fruit development of Physalis floridana
	Zhao, Jing 1,2; Gong, Pichang 1; Liu, Hongyan 1,2; Zhang, Mingshu 1,2; He, Chaoying1,2,3
	2021
发表期刊	PLANT MOLECULAR BIOLOGY
ISSN	0167-4412
卷号	107 期号:1-2 页码:101-116
摘要	Key message This work reveals potentially multiple and integrated roles in flower and fruit development of floral C-class MADS-box genes in Physalis. The Physalis fruit features a morphological novelty, the Chinese lantern. Floral C-class MADS-domain AGAMOUS-like (AG-like) proteins can interact with the identified regulators of this novel structure. However, the developmental role of the floral C-class genes is unknown in Physalis. Here, we characterized two AG-like genes from Physalis floridana, designated PFAG1 and PFAG2. The two paralogous genes shared around 61.0% of sequence identity and had similar expression domains, with different expression levels in the floral and berry development. However, the genes had distinct expression patterns in leaf and calyx development. Protein-protein interaction analyses revealed that PFAG1 and PFAG2 could commonly or specifically dimerize with certain floral MADS-domain proteins as well as non-MADS-domain proteins involved in various floral developmental processes. Gene downregulation analyses demonstrated that PFAG1 may repress PFAG2, but PFAG2 did not affect PFAG1. Downregulating PFAG1 led to incomplete floral homeotic variation in the stamens and carpels, and alteration of petal coloration pattern, while downregulating PFAG2 did not result in any floral homeotic variation. PFAG1 affected pollen maturation, while PFAG2 affected female fertility. However, simultaneously downregulating PFAG1 and PFAG2 caused loss of the complete C-function, indicating that the two PFAG genes interact to determine the identity and functionality of androecia and gynoecia organs. Their potential roles in regulating fruit size and the Chinese lantern are also discussed. Our results reveal functional divergence of floral C-class MADS-box genes in Physalis, demonstrating that they may play multiple and integrated roles in flower and fruit development.
关键词	Functional divergence AGAMOUS-like gene Organ identity Male fertility Fruit development Physalis floridana
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1007/s11103-021-01182-4
收录类别	SCI
语种	英语
WOS关键词	MOLECULAR CHARACTERIZATION ; MORPHOLOGICAL NOVELTY ; AGAMOUS SUBFAMILY ; CHINESE LANTERN ; DOWN-REGULATION ; EXPRESSION ; EVOLUTION ; DIVERSIFICATION ; REPRESSION ; LOCUS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000687500700002
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5028
资助机构	National Natural Science Foundation of China [31500190] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB27010106] ; Youth Innovation Promotion Association CAS [2019081]
作者邮箱	chaoying@ibcas.ac.cn
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26292
专题	系统与进化植物学国家重点实验室
作者单位	1.Northwest Normal Univ, Life Sci Coll, Lanzhou 730070, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Jing,Gong, Pichang,Liu, Hongyan,et al. Multiple and integrated functions of floral C-class MADS-box genes in flower and fruit development of Physalis floridana[J]. PLANT MOLECULAR BIOLOGY,2021,107(1-2):101-116.
APA	Zhao, Jing,Gong, Pichang,Liu, Hongyan,Zhang, Mingshu,&He, Chaoying.(2021).Multiple and integrated functions of floral C-class MADS-box genes in flower and fruit development of Physalis floridana.PLANT MOLECULAR BIOLOGY,107(1-2),101-116.
MLA	Zhao, Jing,et al."Multiple and integrated functions of floral C-class MADS-box genes in flower and fruit development of Physalis floridana".PLANT MOLECULAR BIOLOGY 107.1-2(2021):101-116.

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