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Metabolic profiling and gene expression analysis provides insights into flavonoid and anthocyanin metabolism in poplar
Tian, Yuru1; Li, Qianqian1; Rao, Shupei1; Wang, Aike2,3; Zhang, Hechen4; Wang, Liangsheng5; Li, Yue1; Chen, Jinhuan1
2021
发表期刊TREE PHYSIOLOGY
ISSN0829-318X
卷号41期号:6页码:1046-1064
摘要Poplar, a woody perennial model, is a common and widespread tree genus. We cultivated two red leaf poplar varieties from bud mutation of Populus sp. Linn. '2025' (also known as Zhonglin 2025, L2025 for shot): Populus deltoides varieties with bright red leaves (LHY) and completely red leaves (QHY). After measuring total contents of flavonoid, anthocyanin, chlorophyll and carotenoid metabolites, a liquid chromatography-electrospray ionization-tandem mass spectrometry system was used for the relative quantification of widely targeted metabolites in leaves of three poplar varieties. A total of 210 flavonoid metabolites (89 flavones, 40 flavonols, 25 flavanones, 18 anthocyanins, 16 isoflavones, 7 dihydroflavonols, 7 chalcones, 5 proanthocyanidins and 3 other flavonoid metabolites) were identified. Compared with L2025, 48 and 8 flavonoids were more and less abundant, respectively, in LHY, whereas 51 and 9 flavonoids were more and less abundant in QHY, respectively. On the basis of a comprehensive analysis of the metabolic network, gene expression levels were analyzed by deep sequencing to screen for potential reference genes for the red leaves. Most phenylpropanoid biosynthesis pathway-involved genes were differentially expressed among the examined varieties. Gene expression analysis also revealed several potential anthocyanin biosynthesis regulators including three MYB genes. The study results provide new insights into poplar flavonoid metabolites and represent the theoretical basis for future studies on leaf coloration in this model tree species.
关键词anthocyanin flavonoids gene expression leaf coloration metabolic profiling poplar.
学科领域Forestry
DOI10.1093/treephys/tpaa152
收录类别SCI
语种英语
WOS关键词POPULUS ; BIOSYNTHESIS ; ACCUMULATION ; TRANSCRIPTOME ; EVOLUTION ; ISOORIENTIN ; REVEALS ; COLORS ; POTATO ; MODEL
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000671960000012
出版者OXFORD UNIV PRESS
文献子类Article
出版地OXFORD
EISSN1758-4469
资助机构National Natural Science Foundation of China [31670610, 31370597] ; Fundamental Research Funds for the Central University [2016ZCQ05]
作者邮箱chenjh@bjfu.edu.cn
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/26295
专题中科院北方资源植物重点实验室
作者单位1.Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing Adv Innovat Ctr Tree Breeding Mol Design, 35 Qinghua East Rd, Beijing 100083, Peoples R China
2.Beijing Forestry Univ, Natl Engn Lab Tree Breeding, 35 Qinghua East Rd, Beijing 100083, Peoples R China
3.Yucheng Inst Agr Sci, Shangqiu 476000, Henan, Peoples R China
4.Shangqiu Zhongxing Seedling Planting Co Ltd, Shangqiu 476000, Henan, Peoples R China
5.Henan Acad Agr Sci, Hort Res Inst, Zhengzhou 450002, Henan, Peoples R China
6.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, 20 Nanxincun, Beijing 100093, Peoples R China
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Tian, Yuru,Li, Qianqian,Rao, Shupei,et al. Metabolic profiling and gene expression analysis provides insights into flavonoid and anthocyanin metabolism in poplar[J]. TREE PHYSIOLOGY,2021,41(6):1046-1064.
APA Tian, Yuru.,Li, Qianqian.,Rao, Shupei.,Wang, Aike.,Zhang, Hechen.,...&Chen, Jinhuan.(2021).Metabolic profiling and gene expression analysis provides insights into flavonoid and anthocyanin metabolism in poplar.TREE PHYSIOLOGY,41(6),1046-1064.
MLA Tian, Yuru,et al."Metabolic profiling and gene expression analysis provides insights into flavonoid and anthocyanin metabolism in poplar".TREE PHYSIOLOGY 41.6(2021):1046-1064.
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