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| Genome-wide comparative analysis of long-chain acyl-CoA synthetases (LACSs) gene family: A focus on identification, evolution and expression profiling related to lipid synthesis | |
| Ayaz, Asma; Saqib, Saddam1,2; Huang, Haodong; Zaman, Wajid1,2; Lu, Shiyou; Zhao, Huayan | |
| 2021 | |
| 发表期刊 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
 |
| ISSN | 0981-9428 |
| 卷号 | 161页码:1-11 |
| 摘要 | In plants, Long-chain acyl-CoA synthetases (LACSs) play key roles in activating fatty acids to fatty acyl-CoA thioesters, which are then further involved in lipid synthesis and fatty acid catabolism. LACSs have been intensively studied in Arabidopsis, but its evolutionary relationship in green plants is unexplored. In this study, we performed a comprehensive genome-wide analysis of the LACS gene family across green plants followed by phylogenetic clustering analysis, gene structure determination, detection of conserved motifs, gene expression in tissues and subcellular localization. Our results identified LACS genes in 122 plant species including algae, low land plants (i.e., mosses and lycophytes), monocots, and eudicots. In total, 697 sequences were identified, and 629 sequences were selected because of alignment and some duplication errors. The retrieved amino acid sequences ranged from 271 to 1056 residues and diversified in intron/exon patterns in different LACSs. Phylogenetic clustering grouped LACS gene family into six major clades with distinct potential functions. This classification is well supported by examining gene structure and conserved motifs. Also, gene expression analysis and subcellular localization substantiate with clade division in the phylogeny, indicating that the evolutionary pattern is visible in their functionality. Additionally, experimental analysis of lacs2 mutant validated that LACS2 plays key roles in suberin synthesis. Thus, our study not only provides an evolutionary mechanism underlying functional diversification but also lays the foundation for further elucidation of the LACS gene family. |
| 关键词 | LACS Cuticular wax and cutin biosynthesis Fatty acids Genome-wide Phylogenetic analysis |
| 学科领域 | Plant Sciences |
| DOI | 10.1016/j.plaphy.2021.01.042 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | COENZYME-A SYNTHETASE ; FATTY-ACID ; MOLECULAR CHARACTERIZATION ; ENCODES |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000637955100001 |
| 出版者 | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| 文献子类 | Article |
| 出版地 | ISSY-LES-MOULINEAUX |
| EISSN | 1873-2690 |
| 资助机构 | National Key R&D Program of China [2017YFD0600703] |
| 作者邮箱 | asmaayaz@bs.qau.edu.pk ; saddamsaqib.qau@gmail.com ; 396828821@qq.com ; shangla123@gmail.com ; shiyoulu@hubu.edu.cn ; huayanzhao@hubu.edu.cn |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/26303 |
| 专题 | 系统与进化植物学国家重点实验室 |
| 作者单位 | 1.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ayaz, Asma,Saqib, Saddam,Huang, Haodong,et al. Genome-wide comparative analysis of long-chain acyl-CoA synthetases (LACSs) gene family: A focus on identification, evolution and expression profiling related to lipid synthesis[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2021,161:1-11. |
| APA | Ayaz, Asma,Saqib, Saddam,Huang, Haodong,Zaman, Wajid,Lu, Shiyou,&Zhao, Huayan.(2021).Genome-wide comparative analysis of long-chain acyl-CoA synthetases (LACSs) gene family: A focus on identification, evolution and expression profiling related to lipid synthesis.PLANT PHYSIOLOGY AND BIOCHEMISTRY,161,1-11. |
| MLA | Ayaz, Asma,et al."Genome-wide comparative analysis of long-chain acyl-CoA synthetases (LACSs) gene family: A focus on identification, evolution and expression profiling related to lipid synthesis".PLANT PHYSIOLOGY AND BIOCHEMISTRY 161(2021):1-11. |
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| Genome_wide_comparat（7053KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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