Integrative taxonomy of the Selaginella helvetica group based on morphological, molecular and ecological data

doi:10.1002/tax.12565

	Integrative taxonomy of the Selaginella helvetica group based on morphological, molecular and ecological data
	Zhang, Meng-Hua ; Wei, Ran ; Xiang, Qiao-Ping ; Ebihara, Atsushi 2; Zhang, Xian-Chun
	2021
发表期刊	TAXON
ISSN	0040-0262
卷号	70 期号:6 页码:1163-1187
摘要	The Eurasian and Mediterranean Selaginella helvetica group is one of the taxonomically challenging groups in the cosmopolitan lycophyte genus Selaginella. Species of the S. helvetica group are all small plants with lax strobili composed of more or less isomorphic sporophylls (isosporophylls) that are basically non-resupinate. Owing to the similar and minute morphological characteristics, the inapplicability of chloroplast DNA markers for species delimitation and the lack of range-wide sampling, no comprehensive study has been conducted on these species so far. In this study, we performed phylogenetic analyses based on population-scale sampling of 126 individuals, covering the entire geographical distribution and morphological variation of this group. Five chloroplast (rbcL, psbA, atpI, atpF, atpF-H) and two nuclear DNA regions (26S rDNA, pgiC) were used to explore species diversity of this group. The phylogeny inferred from nuclear DNA datasets revealed nine well-supported monophyletic groups, which provided important evidence for species delimitation and called for taxonomic revision of some taxa. However, in the chloroplast phylogeny, individuals of only three species (S. denticulata, S. helvetica, S. tamamontana) formed monophyletic groups, respectively. We further investigated genome size for each species by flow cytometry and evaluated 21 morphological characteristics of 79 herbarium specimens. MaxEnt analysis was performed to predict the potential suitable distribution of each species. As a result of total evidence, 10 taxa were recognized in the S. helvetica group. The delimitation of S. denticulata, S. laxistrobila, S. nipponica, S. pallidissima, S. prostrata, and S. tamamontana was congruent with previous taxonomical treatments. We reinstated S. shensiensis as an independent species and treated S. longistrobilina as its synonym. Selaginella helvetica (s.str.) is confined to Europe and North Asia, former records of it from the Himalaya-Hengduan mountains represent an independent species (S. jiulongensis comb. nov.). The nuclear pgiC phylogeny and flow cytometry evidence indicated that S. pseudonipponica is an allotetraploid with S. shensiensis and S. nipponica as its parents. Our results highlight that nuclear DNA markers rather than chloroplast DNA markers are more informative in delimitation of closely related species in Selaginella.
关键词	26S rDNA pgiC Selaginella Selaginella helvetica group species delimitation
学科领域	Plant Sciences ; Evolutionary Biology
DOI	10.1002/tax.12565
收录类别	SCI
语种	英语
WOS关键词	PHYLOGENETIC-RELATIONSHIPS ; SPECIES DELIMITATION ; NUCLEAR GENES ; CHLOROPLAST ; SEQUENCES ; RATES ; DNA ; CLASSIFICATION ; SAXIFRAGACEAE ; MITOCHONDRIAL
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000692707000001
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1996-8175
资助机构	National Natural Science Foundation of China (NSFC) [31670205, 31811530081]
作者邮箱	zhangxc@ibcas.ac.cn
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26313
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Museum Nat & Sci, Dept Bot, 4-1-1 Amakubo, Tsukuba, Ibaraki 3050005, Japan
推荐引用方式 GB/T 7714	Zhang, Meng-Hua,Wei, Ran,Xiang, Qiao-Ping,et al. Integrative taxonomy of the Selaginella helvetica group based on morphological, molecular and ecological data[J]. TAXON,2021,70(6):1163-1187.
APA	Zhang, Meng-Hua,Wei, Ran,Xiang, Qiao-Ping,Ebihara, Atsushi,&Zhang, Xian-Chun.(2021).Integrative taxonomy of the Selaginella helvetica group based on morphological, molecular and ecological data.TAXON,70(6),1163-1187.
MLA	Zhang, Meng-Hua,et al."Integrative taxonomy of the Selaginella helvetica group based on morphological, molecular and ecological data".TAXON 70.6(2021):1163-1187.

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