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| Light-adapted charge-separated state of photosystem II: structural and functional dynamics of the closed reaction center | |
Sipka, Gabor; Magyar, Melinda; Mezzetti, Alberto1,2; Akhtar, Parveen3; Zhu, Qingjun; Xiao, Yanan; Han, Guangye; Santabarbara, Stefano5; Shen, Jian-Ren ; Lambrev, Petar H.; Garab, Gyozo8
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| 2021 | |
| 发表期刊 | PLANT CELL
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| ISSN | 1040-4651 |
| 卷号 | 33期号:4页码:1286-1302 |
| 摘要 | Photosystem II (PSII) uses solar energy to oxidize water and delivers electrons for life on Earth. The photochemical reaction center of PSII is known to possess two stationary states. In the open state (PSIIO), the absorption of a single photon triggers electron-transfer steps, which convert PSII into the charge-separated closed state (PSIIC). Here, by using steady-state and time-resolved spectroscopic techniques on Spinacia oleracea and Thermosynechococcus vulcanus preparations, we show that additional illumination gradually transforms PSIIC into a light-adapted charge-separated state (PSIIL). The PSIIC-to-PSIIL transition, observed at all temperatures between 80 and 308 K, is responsible for a large part of the variable chlorophyll-a fluorescence (F-v) and is associated with subtle, dark-reversible reorganizations in the core complexes, protein conformational changes at noncryogenic temperatures, and marked variations in the rates of photochemical and photophysical reactions. The build-up of PSIIL requires a series of light-induced events generating rapidly recombining primary radical pairs, spaced by sufficient waiting times between these events-pointing to the roles of local electric-field transients and dielectric relaxation processes. We show that the maximum fluorescence level, F-m, is associated with PSIIL rather than with PSIIC, and thus the F-v/F-m parameter cannot be equated with the quantum efficiency of PSII photochemistry. Our findings resolve the controversies and explain the peculiar features of chlorophyll-a fluorescence kinetics, a tool to monitor the functional activity and the structural-functional plasticity of PSII in different wild-types and mutant organisms and under stress conditions. |
| 学科领域 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| DOI | 10.1093/plcell/koab008 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | PHOTOSYNTHETIC REACTION CENTERS ; CHLOROPHYLL FLUORESCENCE ; ELECTRON-TRANSFER ; PICOSECOND FLUORESCENCE ; CONFORMATIONAL-CHANGES ; INFRARED-SPECTROSCOPY ; VARIABLE FLUORESCENCE ; WAVELENGTH DEPENDENCE ; ENERGY-CONVERSION ; PROTEIN |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000671679400042 |
| 出版者 | OXFORD UNIV PRESS INC |
| 文献子类 | Article |
| 出版地 | CARY |
| EISSN | 1532-298X |
| 资助机构 | Hungarian Ministry of Innovation and Technology, National Research, Development and Innovation Fund (OTKA) [KH-124985, K-128679, PD-121225, NN-124904, 2018-1.2.1-NKP-2018-00009] ; Hungarian Ministry of Innovation and Technology [TET 2018-2.1.14-TET-CN-2018-00004] ; Czech Science Foundation (GACR) [19-13637S] ; Eotvos Lorand Research Network (ELKH KU) [37/2020] ; COST Actions [CM1306, 40047, CA15126, 41468] ; Grant Fondazione Cariplo (CYAO Project) [2016-0667] ; National Key RAMP ; D Program of China [2017YFA0503700] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB17000000] ; National Natural Science Foundation of China [31470339] ; European Union [GINOP-2.3.6-15-2015-00001] ; European Regional Development Fund |
| 作者邮箱 | lambrev.petar@brc.hu ; garab.gyozo@brc.hu |
| 作品OA属性 | Green Published, Green Accepted, Bronze |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/26331 |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Inst Plant Biol, Biol Res Ctr, Szeged, Hungary 2.Univ Paris Saclay, CNRS, CEA, Inst Integrat Biol Cell I2BC, F-91191 Gif Sur Yvette, France 3.Sorbonne Univ, Lab Reactivit Surface, UMR 7197, Paris, France 4.ELI HU Nonprofit Ltd, ELI ALPS, Szeged, Hungary 5.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 6.Natl Res Council Italy, Inst Biophys, Photosynthet Res Unit, Milan, Italy 7.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama, Japan 8.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama, Japan 9.Univ Ostrava, Fac Sci, Ostrava, Czech Republic |
| 推荐引用方式 GB/T 7714 | Sipka, Gabor,Magyar, Melinda,Mezzetti, Alberto,et al. Light-adapted charge-separated state of photosystem II: structural and functional dynamics of the closed reaction center[J]. PLANT CELL,2021,33(4):1286-1302. |
| APA | Sipka, Gabor.,Magyar, Melinda.,Mezzetti, Alberto.,Akhtar, Parveen.,Zhu, Qingjun.,...&Garab, Gyozo.(2021).Light-adapted charge-separated state of photosystem II: structural and functional dynamics of the closed reaction center.PLANT CELL,33(4),1286-1302. |
| MLA | Sipka, Gabor,et al."Light-adapted charge-separated state of photosystem II: structural and functional dynamics of the closed reaction center".PLANT CELL 33.4(2021):1286-1302. |
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| Sipka-2021-Light-ada（1622KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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