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| Defective mitochondrial function by mutation in THICK ALEURONE 1 encoding a mitochon-driontargeted single-stranded DNA-binding protein leads to increased aleurone cell layers and improved nutrition in rice | |
Li, Dong-Qi; Wu, Xiao-Ba; Wang, Hai-Feng; Feng, Xue; Yan, Shi-Juan; Wu, Sheng-Yang; Liu, Jin-Xin; Yao, Xue-Feng; Bai, Ai-Ning; Zhao, Heng; Song, Xiu-Fen; Guo, Lin; Zhang, Shi-Yong; Liu, Chun-Ming
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| 2021 | |
| Source Publication | MOLECULAR PLANT
 |
| ISSN | 1674-2052 |
| Volume | 14Issue:8Pages:1343-1361 |
| Abstract | Cereal endosperm comprises an outer aleurone and an inner starchy endosperm. Although these two tissues have the same developmental origin, they differ in morphology, cell fate, and storage product accumulation, with the mechanism largely unknown. Here, we report the identification and characterization of rice thick aleurone 1 (ta1) mutant that shows an increased number of aleurone cell layers and increased contents of nutritional factors including proteins, lipids, vitamins, dietary fibers, and micronutrients. We identified that the TA1 gene, which is expressed in embryo, aleurone, and subaleurone in caryopses, encodes a mitochondrion-targeted protein with single-stranded DNA-binding activity named OsmtSSB1. Cytological analyses revealed that the increased aleurone cell layers in ta1 originate from a developmental switch of subaleurone toward aleurone instead of starchy endosperm in the wild type. We found that TA1/OsmtSSB1 interacts with mitochondrial DNA recombinase RECA3 and DNA helicase TWINKLE, and down-regulation of RECA3 or TWINKLE also leads to ta1-like phenotypes. We further showed that mutation in TA1/OsmtSSB1 causes elevated illegitimate recombinations in the mitochondrial genome, altered mitochondrial morphology, and compromised energy supply, suggesting that the OsmtSSB1-mediated mitochondrial function plays a critical role in subaleurone cell-fate determination in rice. |
| Keyword | rice thick aleurone cell-fate determination nutritional improvement mitochondria |
| Subject Area | Biochemistry & Molecular Biology ; Plant Sciences |
| DOI | 10.1016/j.molp.2021.05.016 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | ORYZA-SATIVA-L ; MAIZE ENDOSPERM ; TRANSCRIPTION FACTORS ; ALTERNATIVE OXIDASE ; EXPRESSION ANALYSIS ; MOLECULAR-CLONING ; GENE-EXPRESSION ; DYNAMIC CHANGES ; OPAQUE-2 MAIZE ; CARYOPSIS |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000681059100019 |
| Publisher | CELL PRESS |
| Subtype | Article |
| Publication Place | CAMBRIDGE |
| EISSN | 1752-9867 |
| Funding Organization | Chinese Academy of Sciences Innovation Project Molecular Modules for Breeding Design'' [XDA24010402] ; Beijing Municipal Science and Technology Commission Project [Z181100002418010] ; CAS-CSIRO Bilateral Collaboration Project [151111KYSB20180049] |
| Corresponding Author Email | cmliu@ibcas.ac.cn |
| OA | Bronze |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/26335 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.[Li, Dong-Qi 2.Wu, Xiao-Ba 3.Feng, Xue 4.Wu, Sheng-Yang 5.Liu, Jin-Xin 6.Yao, Xue-Feng 7.Bai, Ai-Ning 8.Song, Xiu-Fen 9.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 10.Univ Chinese Acad Sci, Beijing 100864, Peoples R China 11.[Wang, Hai-Feng 12.Shandong Acad Agr Sci, Rice Res Inst, Jinan 250100, Peoples R China 13.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Key Lab Crop Germplasm Resources Preser, Guangzhou 510640, Peoples R China 14.[Zhao, Heng 15.Guo, Lin 16.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 17.Peking Univ, Sch Adv Agr Sci, Beijing 100871, Peoples R China 18.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Dong-Qi,Wu, Xiao-Ba,Wang, Hai-Feng,et al. Defective mitochondrial function by mutation in THICK ALEURONE 1 encoding a mitochon-driontargeted single-stranded DNA-binding protein leads to increased aleurone cell layers and improved nutrition in rice[J]. MOLECULAR PLANT,2021,14(8):1343-1361. |
| APA | Li, Dong-Qi.,Wu, Xiao-Ba.,Wang, Hai-Feng.,Feng, Xue.,Yan, Shi-Juan.,...&Liu, Chun-Ming.(2021).Defective mitochondrial function by mutation in THICK ALEURONE 1 encoding a mitochon-driontargeted single-stranded DNA-binding protein leads to increased aleurone cell layers and improved nutrition in rice.MOLECULAR PLANT,14(8),1343-1361. |
| MLA | Li, Dong-Qi,et al."Defective mitochondrial function by mutation in THICK ALEURONE 1 encoding a mitochon-driontargeted single-stranded DNA-binding protein leads to increased aleurone cell layers and improved nutrition in rice".MOLECULAR PLANT 14.8(2021):1343-1361. |
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| File Name/Size | DocType | Version | Access | License | ||
| Applet_2022-4-17_165（2776KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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