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Cytosine methylation of the FWA promoter promotes direct in vitro shoot regeneration in Arabidopsis thaliana
Dai, Xuehuan; Wang, Jing; Song, Yuguang1; Liu, Zhenhua2; Xue, Tao3; Qiao, Meng; Yu, Yanchong4; Xin, Wei5; Xiang, Fengning
2021
发表期刊JOURNAL OF INTEGRATIVE PLANT BIOLOGY
ISSN1672-9072
卷号63期号:8页码:1491-1504
摘要Epigenetic modifications within promoter sequences can act as regulators of gene expression. Shoot regeneration is influenced by both DNA methylation and histone methylation, but the mechanistic basis of this regulation is obscure. Here, we identified 218 genes related to the regeneration capacity of callus that were differentially transcribed between regenerable calli (RC) and non-regenerable calli (NRC) in Arabidopsis thaliana. An analysis of the promoters of five of the differentially expressed genes (FWA, ACC1, TFL1, MAX3, and GRP3) pointed to an inverse relationship between cytosine methylation and transcription. The FWA promoter was demethylated and highly expressed in NRC, whereas it was methylated and expressed at low levels in RC. Explants of the hypomethylation mutants fwa-1 and fwa-2 showed strong levels of FWA expression and regenerated less readily than the wild type, suggesting that FWA inhibits direct in vitro shoot regeneration. WUSCHEL-RELATED HOMEOBOX 9 (WOX9), which is required for shoot apical meristem formation, was directly repressed by FWA. Overexpressing WOX9 partly rescued the shoot regeneration defect of fwa-2 plants. These findings suggest that cytosine methylation of the FWA promoter forms part of the regulatory system governing callus regenerability and direct in vitro shoot regeneration.
关键词DNA methylation epigenetic modification FLOWERING WAGENINGEN (FWA) shoot regeneration WOX9
学科领域Biochemistry & Molecular Biology ; Plant Sciences
DOI10.1111/jipb.13156
收录类别SCI
语种英语
WOS关键词LATE-FLOWERING PHENOTYPE ; STEM-CELL FATE ; DNA METHYLATION ; GENE-EXPRESSION ; ORGANOGENESIS ; EMBRYOGENESIS ; COMPETENCE ; MUTANTS ; ALLELES ; WUSCHEL
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000686294800009
出版者WILEY
文献子类Article
出版地HOBOKEN
EISSN1744-7909
资助机构National Natural Science Foundation of China [U1906203, 31770317, 31970189] ; National Transgenic Project of China [2016ZX08010002-009, 2018ZX08009-14B] ; China Postdoctoral Science Foundation [2019M662331]
作者邮箱xfn0990@sdu.edu.cn
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/26345
专题中科院植物分子生理学重点实验室
作者单位1.Shandong Univ, Sch Life Sci, Key Lab Plant Dev & Environm Adaptat Biol, Minist Educ, Qingdao 266237, Peoples R China
2.Qufu Normal Univ, Qufu 273165, Shandong, Peoples R China
3.Shandong Univ Tradit Chinese Med, Jinan 250355, Peoples R China
4.Huaibei Normal Univ, Huaibei 235000, Peoples R China
5.Qingdao Agr Univ, Qingdao 266109, Peoples R China
6.Chinese Acad Sci, Inst Bot, Beijing 100101, Peoples R China
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Dai, Xuehuan,Wang, Jing,Song, Yuguang,et al. Cytosine methylation of the FWA promoter promotes direct in vitro shoot regeneration in Arabidopsis thaliana[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2021,63(8):1491-1504.
APA Dai, Xuehuan.,Wang, Jing.,Song, Yuguang.,Liu, Zhenhua.,Xue, Tao.,...&Xiang, Fengning.(2021).Cytosine methylation of the FWA promoter promotes direct in vitro shoot regeneration in Arabidopsis thaliana.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,63(8),1491-1504.
MLA Dai, Xuehuan,et al."Cytosine methylation of the FWA promoter promotes direct in vitro shoot regeneration in Arabidopsis thaliana".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 63.8(2021):1491-1504.
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