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The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat
Xu, Shujuan1; Dong, Qi1; Deng, Min1,4; Lin, Dexing1,5; Xiao, Jun4,7; Cheng, Peilei6; Xing, Lijing; Niu, Yuda; Gao, Caixia5; Zhang, Wenhao1,3; Xu, Yunyuan2; Chong, Kang1,2
2021
发表期刊MOLECULAR PLANT
ISSN1674-2052
卷号14期号:9页码:1525-1538
摘要Vernalization is a physiological process in which prolonged cold exposure establishes flowering competence in winter plants. In hexaploid wheat, TaVRN1 is a cold-induced key regulator that accelerates floral transition. However, the molecular mechanism underlying the gradual activation of TaVRN1 during the vernalization process remains unknown. In this study, we identified the novel transcript VAS (TaVRN1 alternative splicing) as a non-coding RNA derived from the sense strand of the TaVRN1 gene only in winter wheat, which regulates TaVRN1 transcription for flowering. VAS was induced during the early period of vernalization, and its overexpression promoted TaVRN1 expression to accelerate flowering in winter wheat. VAS physically associates with TaRF2b and facilitates docking of the TaRF2b-TaRF2a complex at the TaVRN1 promoter during the middle period of vernalization. TaRF2b recognizes the Sp1 motif within the TaVRN1 proximal promoter region, which is gradually exposed along with the disruption of a loop structure at the TaVRN1 locus during vernalization, to activate the transcription of TaVRN1. The tarf2b mutants exhibited delayed flowering, whereas transgenic wheat lines overexpressing TaRF2b showed earlier flowering. Taken together, our data reveal a distinct regulatory mechanism by which a long non-coding RNA facilitates the transcription factor targeting to regulate wheat flowering, providing novel insights into the vernalization process and a potential target for wheat genetic improvement.
关键词winter wheat vernalization flowering long non-coding RNA chromosome loop
学科领域Biochemistry & Molecular Biology ; Plant Sciences
DOI10.1016/j.molp.2021.05.026
收录类别SCI
语种英语
WOS关键词SPRING GROWTH HABIT ; ALLELIC VARIATION ; FLC ; RF2A ; GENE ; RESPONSES ; ARABIDOPSIS ; REPRESSOR ; PROTEIN ; COOLAIR
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000692963200018
出版者CELL PRESS
文献子类Article
出版地CAMBRIDGE
EISSN1752-9867
资助机构NSFC [31788103] ; National Natural Science Foundation of China [31970529] ; China Postdoctoral Science Foundation [2019M650892]
作者邮箱chongk@ibcas.ac.cn
作品OA属性Bronze
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/26348
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100093, Peoples R China
4.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China
5.Chinese Acad Sci, Innovat Acad Seed Design, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
6.Chinese Acad Sci, Innovat Acad Seed Design, Ctr Genome Editing, Inst Genet & Dev Biol,State Key Lab Plant Cell &, Beijing 100101, Peoples R China
7.Nanjing Agr Univ, Coll Hort, Key Lab Landscaping, Minist Agr, Nanjing 210095, Peoples R China
8.Chinese Acad Sci, CAS JIC Ctr Excellence Plant & Microbial Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
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Xu, Shujuan,Dong, Qi,Deng, Min,et al. The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat[J]. MOLECULAR PLANT,2021,14(9):1525-1538.
APA Xu, Shujuan.,Dong, Qi.,Deng, Min.,Lin, Dexing.,Xiao, Jun.,...&Chong, Kang.(2021).The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat.MOLECULAR PLANT,14(9),1525-1538.
MLA Xu, Shujuan,et al."The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat".MOLECULAR PLANT 14.9(2021):1525-1538.
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