Analysis of the changes of electron transfer and heterogeneity of photosystem II in Deg1-reduced Arabidopsis plants

doi:10.1007/s11120-021-00842-2

IB-CAS > 中科院光生物学重点实验室

	Analysis of the changes of electron transfer and heterogeneity of photosystem II in Deg1-reduced Arabidopsis plants
	Wen, Xiaogang 3; Yang, Zhipan ; Ding, Shunhua ; Yang, Huixia ; Zhang, Lixin2 ; Lu, Congming 1; Lu, Qingtao
	2021
发表期刊	PHOTOSYNTHESIS RESEARCH
ISSN	0166-8595
卷号	150 期号:1-3 页码:159-177
摘要	Deg1 protease functions in protease and chaperone of PSII complex components, but few works were performed to study the effects of Deg1 on electron transport activities on the donor and acceptor side of PSII and its correlation with the photoprotection of PSII during photoinhibition. Therefore, we performed systematic and comprehensive investigations of electron transfers on the donor and acceptor sides of photosystem II (PSII) in the Deg1-reduced transgenic lines deg1-2 and deg1-4. Both the maximal quantum efficiency of PSII photochemistry (F-v/F-m) and the actual PSII efficiency (phi(PSII)) decreased significantly in the transgenic plants. Increases in nonphotochemical quenching (NPQ) and the dissipated energy flux per reaction center (DI0/RC) were also shown in the transgenic plants. Along with the decreased D1, CP47, and CP43 content, these results suggested photoinhibition under growth light conditions in transgenic plants. Decreased Deg1 caused inhibition of electron transfer on the PSII reducing side, leading to a decline in the number of Q(B)-reducing centers and accumulation of Q(B)-nonreducing centers. The Tm of the Q band shifted from 5.7 degrees C in the wild-type plant to 10.4 degrees C and 14.2 degrees C in the deg1-2 and deg1-4 plants, respectively, indicating an increase in the stability of S(2)Q(A)( over bar ) in transgenic plants. PSII alpha in the transgenic plants largely reduced, while PSII beta and PSII gamma increased with the decline in the Deg1 levels in transgenic plants suggesting PSII alpha centers gradually converted into PSII beta and PSII gamma centers in the transgenic plants. Besides, the connectivity of PSII alpha and PSII beta was downregulated in transgenic plants. Our results reveal that downregulation of Deg1 protein levels induced photoinhibition in transgenic plants, leading to loss of PSII activities on both the donor and acceptor sides in transgenic plants. These results give a new insight into the regulation role of Deg1 in PSII electron transport.
关键词	Deg1 Photosystem II Chlorophyll Fluorescence Thermoluminescence PSII heterogeneity Electron transfer
学科领域	Plant Sciences
DOI	10.1007/s11120-021-00842-2
收录类别	SCI
语种	英语
WOS关键词	FLUORESCENCE INDUCTION CURVE ; PHOTODAMAGED D1 PROTEIN ; CHLOROPHYLL-FLUORESCENCE ; DEG PROTEASES ; CHLAMYDOMONAS-REINHARDTII ; MOLECULAR-MECHANISMS ; QUALITY CONTROL ; REPAIR CYCLE ; ANTENNA SIZE ; PHOTOINHIBITION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000651022800001
出版者	SPRINGER
Special Issue	SI
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5079
资助机构	National Key Research and Development Program of China [2020YFA0907602] ; Ministry of Agriculture of the People's Republic of China [2016ZX08009-003-005] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA24010203, XDB17030100]
作者邮箱	wenxg@ibcas.ac.cn ; yangzp@ibcas.ac.cn ; dshhua@ibcas.ac.cn ; hxyang@ibcas.ac.cn ; zhanglixin@henu.edu.cn ; cmlu@sdau.edu.cn ; lu_qingtao@ibcas.ac.cn
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26354
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China 3.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, 85 Minglun St, Kaifeng 475001, Peoples R China 4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wen, Xiaogang,Yang, Zhipan,Ding, Shunhua,et al. Analysis of the changes of electron transfer and heterogeneity of photosystem II in Deg1-reduced Arabidopsis plants[J]. PHOTOSYNTHESIS RESEARCH,2021,150(1-3):159-177.
APA	Wen, Xiaogang.,Yang, Zhipan.,Ding, Shunhua.,Yang, Huixia.,Zhang, Lixin.,...&Lu, Qingtao.(2021).Analysis of the changes of electron transfer and heterogeneity of photosystem II in Deg1-reduced Arabidopsis plants.PHOTOSYNTHESIS RESEARCH,150(1-3),159-177.
MLA	Wen, Xiaogang,et al."Analysis of the changes of electron transfer and heterogeneity of photosystem II in Deg1-reduced Arabidopsis plants".PHOTOSYNTHESIS RESEARCH 150.1-3(2021):159-177.

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