GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response

doi:10.1093/plphys/kiab142

	GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response
	Wang, Zemin 1,2; Wong, Darren Chern Jan 3; Wang, Yi 1,2; Xu, Guangzhao 1,2; Ren, Chong 1; Liu, Yanfei 1,2; Kuang, Yangfu 1,2; Fan, Peige 1,4; Li, Shaohua1 ; Xin, Haiping 5,6; Liang, Zhenchang 1
	2021
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	186 期号:3 页码:1660-1678
摘要	Cultivated grapevine (Vitis) is a highly valued horticultural crop, and cold stress affects its growth and productivity. Wild Amur grape (Vitis amurensis) PAT1 (Phytochrome A signal transduction 1, VaPAT1) is induced by low temperature, and ectopic expression of VaPAT1 enhances cold tolerance in Arabidopsis (Arabidopsis thaliana). However, little is known about the molecular mechanism of VaPAT1 during the cold stress response in grapevine. Here, we confirmed the overexpression of VaPAT1 in transformed grape calli enhanced cold tolerance. Yeast two-hybrid and bimolecular fluorescence complementation assays highlighted an interaction between VaPAT1 with INDETERMINATE-DOMAIN 3 (VaIDD3). A role of VaIDD3 in cold tolerance was also indicated. Transcriptome analysis revealed VaPAT1 and VaIDD3 overexpression and cold treatment coordinately modulate the expression of stress-related genes including lipoxygenase 3 (LOX3), a gene encoding a key jasmonate biosynthesis enzyme. Co-expression network analysis indicated LOX3 might be a downstream target of VaPAT1. Both electrophoretic mobility shift and dual luciferase reporter assays showed the VaPAT1-IDD3 complex binds to the IDD-box (AGACAAA) in the VaLOX3 promoter to activate its expression. Overexpression of both VaPAT1 and VaIDD3 increased the transcription of VaLOX3 and JA levels in transgenic grape calli. Conversely, VaPAT1-SRDX (dominant repression) and CRISPR/Cas9-mediated mutagenesis of PAT1-ED causing the loss of the C-terminus in grape calli dramatically prohibited the accumulation of VaLOX3 and JA levels during cold treatment. Together, these findings point to a pivotal role of VaPAT1 in the cold stress response in grape by regulating JA biosynthesis.
学科领域	Plant Sciences
DOI	10.1093/plphys/kiab142
收录类别	SCI
语种	英语
WOS关键词	GENE-EXPRESSION ; SIGNAL-TRANSDUCTION ; VITIS-AMURENSIS ; SALICYLIC-ACID ; DNA-BINDING ; TOLERANCE ; PROTEIN ; DROUGHT ; FAMILY ; OVEREXPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000674744100030
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-2548
资助机构	National Key Research and Development Program [2019YFD1002501] ; National Science Foundation of China [32025032]
作者邮箱	zl249@ibcas.ac.cn
作品OA属性	Green Published
引用统计	被引频次：43[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26360
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Innovat Acad Seed Design, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 4.Australian Natl Univ, Res Sch Biol, Dept Ecol & Evolut, Acton, ACT 2601, Australia 5.China Wine Ind Technol Inst, Yinchuan 750021, Ningxia, Peoples R China 6.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Peoples R China 7.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Wang, Zemin,Wong, Darren Chern Jan,Wang, Yi,et al. GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response[J]. PLANT PHYSIOLOGY,2021,186(3):1660-1678.
APA	Wang, Zemin.,Wong, Darren Chern Jan.,Wang, Yi.,Xu, Guangzhao.,Ren, Chong.,...&Liang, Zhenchang.(2021).GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response.PLANT PHYSIOLOGY,186(3),1660-1678.
MLA	Wang, Zemin,et al."GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response".PLANT PHYSIOLOGY 186.3(2021):1660-1678.

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