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| A unique photosystem I reaction center from a chlorophyll d-containing cyanobacterium Acaryochloris marina | |
Xu, Caihuang; Zhu, Qingjun3; Chen, Jing-Hua; Shen, Liangliang3; Yi, Xiaohan1; Huang, Zihui1; Wang, Wenda; Chen, Min5; Kuang, Tingyun; Shen, Jian-Ren ; Zhang, Xing1,4; Han, Guangye2
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| 2021 | |
| 发表期刊 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
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| ISSN | 1672-9072 |
| 卷号 | 63期号:10页码:1740-1752 |
| 摘要 | Photosystem I (PSI) is a large protein supercomplex that catalyzes the light-dependent oxidation of plastocyanin (or cytochrome c(6)) and the reduction of ferredoxin. This catalytic reaction is realized by a transmembrane electron transfer chain consisting of primary electron donor (a special chlorophyll (Chl) pair) and electron acceptors A(0), A(1), and three Fe4S4 clusters, F-X, F-A, and F-B. Here we report the PSI structure from a Chl d-dominated cyanobacterium Acaryochloris marina at 3.3 angstrom resolution obtained by single-particle cryo-electron microscopy. The A. marina PSI exists as a trimer with three identical monomers. Surprisingly, the structure reveals a unique composition of electron transfer chain in which the primary electron acceptor A(0) is composed of two pheophytin a rather than Chl a found in any other well-known PSI structures. A novel subunit Psa27 is observed in the A. marina PSI structure. In addition, 77 Chls, 13 alpha-carotenes, two phylloquinones, three Fe-S clusters, two phosphatidyl glycerols, and one monogalactosyl-diglyceride were identified in each PSI monomer. Our results provide a structural basis for deciphering the mechanism of photosynthesis in a PSI complex with Chl d as the dominating pigments and absorbing far-red light. |
| 关键词 | A marina chlorophyll d cryoelectron microscopy pheophytin a photosynthesis photosystem I reaction center |
| 学科领域 | Biochemistry & Molecular Biology ; Plant Sciences |
| DOI | 10.1111/jipb.13113 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | ENERGY-TRANSFER PATHWAYS ; PRIMARY ELECTRON-DONOR ; CRYO-EM STRUCTURE ; PHOTOSYNTHETIC PROKARYOTE ; PIGMENT COMPOSITION ; CHARGE SEPARATION ; STRUCTURAL BASIS ; ANTENNA ; SUPERCOMPLEX ; EVOLUTION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000665208700001 |
| 出版者 | WILEY |
| 文献子类 | Article |
| 出版地 | HOBOKEN |
| EISSN | 1744-7909 |
| 资助机构 | National Key R&D Program of China [2020YFA0907600, 2017YFA0503700, 2017YFA0504803, 2018YFA0507700, 2019YFA0906300] ; Strategic Priority Research Program of CAS [XDA27050402, XDB17000000] ; Chinese Academy of Sciences Key Research Program of Frontier Sciences [QYZDY-SSW-SMC003] ; Youth Innovation Promotion Association of CAS [2020081] ; CAS Interdisciplinary Innovation Team [JCTD-2020-06] ; Fundamental Research Funds for the Central Universities [2018XZZX001-13] |
| 作者邮箱 | shen@cc.okayama-u.ac.jp ; xzhang1999@zju.edu.cn ; hanguangye@ibcas.ac.cn |
| 作品OA属性 | hybrid |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/26367 |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Zhejiang Univ, Dept Biophys, Sch Med, Sir Run Run Shaw Hosp, Hangzhou 310058, Peoples R China 2.Zhejiang Univ, Dept Pathol, Sch Med, Sir Run Run Shaw Hosp, Hangzhou 310058, Peoples R China 3.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 5.Zhejiang Univ, Ctr Cryoelectron Microscopy, Sch Med, Hangzhou 310058, Peoples R China 6.Univ Sydney, Sch Life & Environm Sci, Fac Sci, Sydney, NSW 2006, Australia 7.Okayama Univ, Inst Interdisciplinary Sci, Div Photosynth & Struct Biol, Okayama 7008530, Japan 8.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan |
| 推荐引用方式 GB/T 7714 | Xu, Caihuang,Zhu, Qingjun,Chen, Jing-Hua,et al. A unique photosystem I reaction center from a chlorophyll d-containing cyanobacterium Acaryochloris marina[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2021,63(10):1740-1752. |
| APA | Xu, Caihuang.,Zhu, Qingjun.,Chen, Jing-Hua.,Shen, Liangliang.,Yi, Xiaohan.,...&Han, Guangye.(2021).A unique photosystem I reaction center from a chlorophyll d-containing cyanobacterium Acaryochloris marina.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,63(10),1740-1752. |
| MLA | Xu, Caihuang,et al."A unique photosystem I reaction center from a chlorophyll d-containing cyanobacterium Acaryochloris marina".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 63.10(2021):1740-1752. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 34002536.pdf（7735KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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