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Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4
Hu, Jiu-Hui; Chang, Jing-Wen; Xu, Tao; Wang, Jia1; Wang, Xiao2; Lin, Rongcheng1; Duanmu, Deqiang3; Liu, Lin2
2021
发表期刊PROTEIN SCIENCE
ISSN0961-8368
卷号30期号:10页码:2083-2091
摘要The chlorophyll biosynthesis regulator GENOMES UNCOUPLED 4 (GUN4) is conserved in nearly all oxygenic photosynthetic organisms. Recently, GUN4 has been found to be able to bind the linear tetrapyrroles (bilins) and stimulate the magnesium chelatase activity in the unicellular green alga Chlamydomonas reinhardtii. Here, we characterize GUN4 proteins from Arabidopsis thaliana and the cyanobacterium Synechocystis sp. PCC 6803 for their ability to bind bilins, and present the crystal structures of Synechocystis GUN4 in biliverdin-bound, phycocyanobilin-bound, and phytochromobilin-bound forms at the resolutions of 1.05, 1.10, and 1.70 angstrom, respectively. These linear molecules adopt a cyclic-helical conformation, and bind more tightly than planar porphyrins to the tetrapyrrole-binding pocket of GUN4. Based on structural comparison, we propose a working model of GUN4 in regulation of tetrapyrrole biosynthetic pathway, and address the role of the bilin-bound GUN4 in retrograde signaling.
关键词biliverdin phycocyanobilin retrograde signaling tetrapyrrole
学科领域Biochemistry & Molecular Biology
DOI10.1002/pro.4164
收录类别SCI
语种英语
WOS关键词GENOMES UNCOUPLED 4 ; MG-CHELATASE ; STRUCTURE REFINEMENT ; GENE-EXPRESSION ; SUBUNIT ; PROTEIN ; PROMOTE ; MUTANT ; MODEL
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000686926700001
出版者WILEY
文献子类Article
出版地HOBOKEN
EISSN1469-896X
资助机构Anhui Provincial Wan Jiang Scholars Program ; Ministry of Science and Technology of the People's Republic of China [2017YFA0503703]
作者邮箱liulin@ahu.edu.cn
作品OA属性Green Published
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/26390
专题中科院光生物学重点实验室
作者单位1.Anhui Univ, Sch Life Sci, 111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China
3.Anhui Univ, Anhui Key Lab Modern Biomfg, Hefei, Peoples R China
4.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
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GB/T 7714
Hu, Jiu-Hui,Chang, Jing-Wen,Xu, Tao,et al. Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4[J]. PROTEIN SCIENCE,2021,30(10):2083-2091.
APA Hu, Jiu-Hui.,Chang, Jing-Wen.,Xu, Tao.,Wang, Jia.,Wang, Xiao.,...&Liu, Lin.(2021).Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4.PROTEIN SCIENCE,30(10),2083-2091.
MLA Hu, Jiu-Hui,et al."Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4".PROTEIN SCIENCE 30.10(2021):2083-2091.
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