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Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4 | |
Hu, Jiu-Hui; Chang, Jing-Wen; Xu, Tao; Wang, Jia1; Wang, Xiao2; Lin, Rongcheng1; Duanmu, Deqiang3; Liu, Lin2 | |
2021 | |
发表期刊 | PROTEIN SCIENCE |
ISSN | 0961-8368 |
卷号 | 30期号:10页码:2083-2091 |
摘要 | The chlorophyll biosynthesis regulator GENOMES UNCOUPLED 4 (GUN4) is conserved in nearly all oxygenic photosynthetic organisms. Recently, GUN4 has been found to be able to bind the linear tetrapyrroles (bilins) and stimulate the magnesium chelatase activity in the unicellular green alga Chlamydomonas reinhardtii. Here, we characterize GUN4 proteins from Arabidopsis thaliana and the cyanobacterium Synechocystis sp. PCC 6803 for their ability to bind bilins, and present the crystal structures of Synechocystis GUN4 in biliverdin-bound, phycocyanobilin-bound, and phytochromobilin-bound forms at the resolutions of 1.05, 1.10, and 1.70 angstrom, respectively. These linear molecules adopt a cyclic-helical conformation, and bind more tightly than planar porphyrins to the tetrapyrrole-binding pocket of GUN4. Based on structural comparison, we propose a working model of GUN4 in regulation of tetrapyrrole biosynthetic pathway, and address the role of the bilin-bound GUN4 in retrograde signaling. |
关键词 | biliverdin phycocyanobilin retrograde signaling tetrapyrrole |
学科领域 | Biochemistry & Molecular Biology |
DOI | 10.1002/pro.4164 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | GENOMES UNCOUPLED 4 ; MG-CHELATASE ; STRUCTURE REFINEMENT ; GENE-EXPRESSION ; SUBUNIT ; PROTEIN ; PROMOTE ; MUTANT ; MODEL |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000686926700001 |
出版者 | WILEY |
文献子类 | Article |
出版地 | HOBOKEN |
EISSN | 1469-896X |
资助机构 | Anhui Provincial Wan Jiang Scholars Program ; Ministry of Science and Technology of the People's Republic of China [2017YFA0503703] |
作者邮箱 | liulin@ahu.edu.cn |
作品OA属性 | Green Published |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/26390 |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Anhui Univ, Sch Life Sci, 111 Jiulong Rd, Hefei 230601, Anhui, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 3.Anhui Univ, Anhui Key Lab Modern Biomfg, Hefei, Peoples R China 4.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China |
推荐引用方式 GB/T 7714 | Hu, Jiu-Hui,Chang, Jing-Wen,Xu, Tao,et al. Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4[J]. PROTEIN SCIENCE,2021,30(10):2083-2091. |
APA | Hu, Jiu-Hui.,Chang, Jing-Wen.,Xu, Tao.,Wang, Jia.,Wang, Xiao.,...&Liu, Lin.(2021).Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4.PROTEIN SCIENCE,30(10),2083-2091. |
MLA | Hu, Jiu-Hui,et al."Structural basis of bilin binding by the chlorophyll biosynthesis regulator GUN4".PROTEIN SCIENCE 30.10(2021):2083-2091. |
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Hu-2021-Structural b(2442KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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