Construction and application of easy-to-detect cyanobacteria with vp28 gene

doi:10.1007/s10811-021-02414-2

IB-CAS > 中科院光生物学重点实验室

	Construction and application of easy-to-detect cyanobacteria with vp28 gene
	Zhuang, Min-Min ; Peng, Wen ; Xu, Yang ; Jia, Xiao-Hui ; Shi, Ding-Ji ; He, Pei-Min ; Jia, Rui 4
	2021
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	33 期号:4 页码:2341-2348
摘要	White Spot Syndrome Virus (WSSV) is the most harmful and pathogenic infectious agent in shrimp aquaculture. Vp28, a protein used to protect hosts against this virus, has successfully been expressed in nine hosts since 2004; however, to date, it has not been used commercially. We constructed transgenic Synechococcus elongatus PCC 7942 expressing vp28 and enhanced green fluorescent protein (egfp) genes simultaneously to enable the easy detection of vp28 expression. GFP has been expressed in cyanobacteria previously and was used in this study to construct transgenic cyanobacteria that can easily be detected. The optimal growth conditions for the transgenic cyanobacteria were determined using a liquid-phase oxygen electrode. At the optimum light intensity, the net photosynthetic rate of the transgenic cyanobacteria increased by 12.6% compared with that of the wild-type. Furthermore, Synechococcus sp. PCC 7942 harboring vp28 and vp28-egfp were fed to shrimp in order to protect them against WSSV infection. The cumulative survival rate of the groups fed with transgenic cyanobacteria was 75%.
关键词	vp28 gene egfp gene Synechococcus elongatus PCC 7942 WSSV
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI	10.1007/s10811-021-02414-2
收录类别	SCI
语种	英语
WOS关键词	SPOT SYNDROME VIRUS ; VECTORS ; BIOLOGY ; DESIGN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000655833100001
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5176
资助机构	National Key R&D Program of China [2019YFC0312604] ; shanghai Agriculture Science and Technology Innovation Project (2017) [1-13] ; Shanghai Science and Technology Commission Innovation Project [17391902200] ; National High Technology Research and Development Program of China (863 Program) [2014AA093506]
作者邮箱	993481987@qq.com ; 199610pw@163.com ; stuart0131@qq.com ; xhjia@bnu.edu.cn ; cyanoshi@163.com ; pmhe@shou.edu.cn ; rjia@shou.edu.cn
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26402
专题	中科院光生物学重点实验室
作者单位	1.East China Normal Univ, Shanghai 200241, Peoples R China 2.[Peng, Wen 3.Xu, Yang 4.He, Pei-Min 5.Shanghai Ocean Univ, Coll Marine Ecol & Environm, Shanghai 201306, Peoples R China 6.Beijing Normal Univ, Beijing 100875, Peoples R China 7.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhuang, Min-Min,Peng, Wen,Xu, Yang,et al. Construction and application of easy-to-detect cyanobacteria with vp28 gene[J]. JOURNAL OF APPLIED PHYCOLOGY,2021,33(4):2341-2348.
APA	Zhuang, Min-Min.,Peng, Wen.,Xu, Yang.,Jia, Xiao-Hui.,Shi, Ding-Ji.,...&Jia, Rui.(2021).Construction and application of easy-to-detect cyanobacteria with vp28 gene.JOURNAL OF APPLIED PHYCOLOGY,33(4),2341-2348.
MLA	Zhuang, Min-Min,et al."Construction and application of easy-to-detect cyanobacteria with vp28 gene".JOURNAL OF APPLIED PHYCOLOGY 33.4(2021):2341-2348.

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