Cryo-EM Structure of the Photosynthetic LH1-RC Complex from Rhodospirillum rubrum

doi:10.1021/acs.biochem.1c00360

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	Cryo-EM Structure of the Photosynthetic LH1-RC Complex from Rhodospirillum rubrum
	Tani, Kazutoshi 5; Kanno, Ryo ; Ji, Xuan-Cheng ; Hall, Malgorzata ; Yu, Long-Jiang; Kimura, Yukihiro 3; Madigan, Michael T.; Mizoguchi, Akira 5; Humbel, Bruno M.; Wang-Otomo, Zheng-Yu
	2021
发表期刊	BIOCHEMISTRY
ISSN	0006-2960
卷号	60 期号:32 页码:2483-2491
摘要	Rhodospirillum (Rsp.) rubrum is one of the most widely used model organisms in bacterial photosynthesis. This purple phototroph is characterized by the presence of both rhodoquinone (RQ) and ubiquinone as electron carriers and bacteriochlorophyll (BChl) a esterified at the propionic acid side chain by geranylgeraniol (BChl a(G)) instead of phytol. Despite intensive efforts, the structure of the light-harvesting-reaction center (LH1-RC) core complex from Rsp. rubrum remains at low resolutions. Using cryo-EM, here we present a robust new view of the Rsp. rubrum LH1-RC at 2.76 angstrom resolution. The LH1 complex forms a closed, slightly elliptical ring structure with 16 alpha beta-polypeptides surrounding the RC. Our biochemical analysis detected RQ molecules in the purified LH1-RC, and the cryo-EM density map specifically positions RQ at the Q(A) site in the RC. The geranylgeraniol side chains of BChl a(G) coordinated by LH1 beta-polypeptides exhibit a highly homologous tail-up conformation that allows for interactions with the bacteriochlorin rings of nearby LH1 alpha-associated BChls a(G). The structure also revealed key protein-protein interactions in both Nand C-terminal regions of the LH1 alpha beta-polypeptides, mainly within a face-to-face structural subunit. Our high-resolution Rsp. rubrum LH1-RC structure provides new insight for evaluating past experimental and computational results obtained with this old organism over many decades and lays the foundation for more detailed exploration of light-energy conversion, quinone transport, and structure-function relationships in this pigment-protein complex.
学科领域	Biochemistry & Molecular Biology
DOI	10.1021/acs.biochem.1c00360
收录类别	SCI
语种	英语
WOS关键词	LIGHT-HARVESTING COMPLEX ; CHEMICALLY SYNTHESIZED POLYPEPTIDES ; IN-VITRO RECONSTITUTION ; SUBUNIT FORM ; B820 SUBUNIT ; BACTERIOCHLOROPHYLL BINDING ; RHODOBACTER-SPHAEROIDES ; MINIMAL REQUIREMENTS ; ALPHA-POLYPEPTIDE ; BETA-POLYPEPTIDE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000687059000005
出版者	AMER CHEMICAL SOC
文献子类	Article
出版地	WASHINGTON
资助机构	Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED [JP21am0101118, 1758, JP21am0101116, 1878, 17am0101116, 18am0101116, 19am0101116, 20am0101116] ; Okinawa Institute of Science and Technology ; National Key R&D Program of China [2019YFA0904600] ; JSPS KAKENHI grant [JP16H04174, JP18H05153, JP20H05086, JP20H02856] ; Takeda Science Foundation ; Kurata Memorial Hitachi Science and Technology Foundation, Japan ; Japanese Cabinet Office
作者邮箱	ktani@doc.medic.mie-u.ac.jp ; wang@ml.ibaraki.ac.jp
作品OA属性	Green Submitted
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26407
专题	中科院光生物学重点实验室
作者单位	1.[Kanno, Ryo; Hall, Malgorzata; Humbel, Bruno M.] Okinawa Inst Sci & Technol Grad Univ OIST, Res Support Div, Imaging Sect, Onna, Okinawa 9040495, Japan 2.Ibaraki Univ, Fac Sci, Mito, Ibaraki 3108512, Japan 3.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photo Biol, Beijing 100093, Peoples R China 4.Kobe Univ, Grad Sch Agr, Dept Agrobiosci, Kobe, Hyogo 6578501, Japan 5.Madigan, Michael T.] Southern Illinois Univ, Sch Biol Sci, Carbondale, IL 62901 USA 6.Mie Univ, Grad Sch Med, Tsu, Mie 5148507, Japan
推荐引用方式 GB/T 7714	Tani, Kazutoshi,Kanno, Ryo,Ji, Xuan-Cheng,et al. Cryo-EM Structure of the Photosynthetic LH1-RC Complex from Rhodospirillum rubrum[J]. BIOCHEMISTRY,2021,60(32):2483-2491.
APA	Tani, Kazutoshi.,Kanno, Ryo.,Ji, Xuan-Cheng.,Hall, Malgorzata.,Yu, Long-Jiang.,...&Wang-Otomo, Zheng-Yu.(2021).Cryo-EM Structure of the Photosynthetic LH1-RC Complex from Rhodospirillum rubrum.BIOCHEMISTRY,60(32),2483-2491.
MLA	Tani, Kazutoshi,et al."Cryo-EM Structure of the Photosynthetic LH1-RC Complex from Rhodospirillum rubrum".BIOCHEMISTRY 60.32(2021):2483-2491.

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