The ubiquitin-interacting motif-type ubiquitin receptor HDR3 interacts with and stabilizes the histone acetyltransferase GW6a to control the grain size in rice

doi:10.1093/plcell/koab194

	The ubiquitin-interacting motif-type ubiquitin receptor HDR3 interacts with and stabilizes the histone acetyltransferase GW6a to control the grain size in rice
	Gao, Qiong ; Zhang, Ning ; Wang, Wei-Qing ; Shen, Shao-Yan ; Bai, Chen ; Song, Xian-Jun
	2021
发表期刊	PLANT CELL
ISSN	1040-4651
卷号	33 期号:10 页码:3331-3347
摘要	For grain crops such as rice (Oryza sativa), grain size substantially affects yield. The histone acetyltransferase GRAIN WEIGHT 6a (GW6a) determines grain size and yield in rice. However, the gene regulatory network underlying GW6a-mediated regulation of grain size has remained elusive. In this study, we show that GW6a interacts with HOMOLOG OF DA1 ON RICE CHROMOSOME 3 (HDR3), a ubiquitin-interacting motif-containing ubiquitin receptor. Transgenic rice plants overexpressing HDR3 produced larger grains, whereas HDR3 knockout lines produce smaller grains compared to the control. Cytological data suggest that HDR3 modulates grain size in a similar manner to GW6a, by altering cell proliferation in spikelet hulls. Mechanistically, HDR3 physically interacts with and stabilizes GW6a in an ubiquitin-dependent manner, delaying protein degradation by the 26S proteasome. The delay in GW6a degradation results in dramatic enhancement of the local acetylation of H3 and H4 histones. Furthermore, RNA sequencing analysis and chromatin immunoprecipitation assays reveal that HDR3 and GW6a bind to the promoters of and modulate a common set of downstream genes. In addition, genetic analysis demonstrates that HDR3 functions in the same genetic pathway as GW6a to regulate the grain size. Therefore, we identified the grain size regulatory module HDR3-GW6a as a potential target for crop yield improvement.
学科领域	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
DOI	10.1093/plcell/koab194
收录类别	SCI
语种	英语
WOS关键词	ORGAN SIZE ; MULTIUBIQUITIN CHAIN ; NATURAL VARIATION ; RARE ALLELE ; BINDING ; YIELD ; DA1 ; WEIGHT ; SEED ; DEGRADATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000711392400015
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-298X
资助机构	Chinese Academy of Sciences [XDA24010101-2] ; National Key Research and Development Program of China [2016YFD0100402] ; National Natural Science Foundation of China [91735302, 91435113, 31471466]
作者邮箱	songxj@ibcas.ac.cn
作品OA属性	Green Published
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26428
专题	中科院植物分子生理学重点实验室
作者单位	1.[Gao, Qiong 2.Zhang, Ning 3.Wang, Wei-Qing 4.Shen, Shao-Yan 5.Bai, Chen 6.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, 20 Nanxincun, Beijing 100093, Peoples R China 7.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China 8.[Zhang, Ning 9.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Gao, Qiong,Zhang, Ning,Wang, Wei-Qing,et al. The ubiquitin-interacting motif-type ubiquitin receptor HDR3 interacts with and stabilizes the histone acetyltransferase GW6a to control the grain size in rice[J]. PLANT CELL,2021,33(10):3331-3347.
APA	Gao, Qiong,Zhang, Ning,Wang, Wei-Qing,Shen, Shao-Yan,Bai, Chen,&Song, Xian-Jun.(2021).The ubiquitin-interacting motif-type ubiquitin receptor HDR3 interacts with and stabilizes the histone acetyltransferase GW6a to control the grain size in rice.PLANT CELL,33(10),3331-3347.
MLA	Gao, Qiong,et al."The ubiquitin-interacting motif-type ubiquitin receptor HDR3 interacts with and stabilizes the histone acetyltransferase GW6a to control the grain size in rice".PLANT CELL 33.10(2021):3331-3347.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Gao-2021-The ubiquit（2166KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文