OsFes1C, a potential nucleotide exchange factor for OsBiP1, is involved in the ER and salt stress responses

doi:10.1093/plphys/kiab263

	OsFes1C, a potential nucleotide exchange factor for OsBiP1, is involved in the ER and salt stress responses
	Qian, Dandan ; Xiong, Shuo 1; Li, Mei 1; Tian, Lihong ; Qu, Le Qing 1
	2021
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	187 期号:1 页码:396-408
摘要	The endoplasmic reticulum (ER) quality control system monitors protein homeostasis and relies on the activity of many molecular chaperones. Binding immunoglobulin protein (BiP) is a major ER luminal chaperone that is involved in most functions of the organelle. BiP activity is tightly regulated by nucleotide exchange factors (NEFs). However, information about NEFs in plants is limited. We obtained a Fes1-like protein (OsFes1C) through isobaric tags for relative and absolute quantitation-based proteomics analysis of ER-stressed rice (Oryza sativa) seeds. Unlike its homologs in yeast and mammals, which are located in the cytosol and respond to heat stress, OsFes1C is an ER membrane protein and responds to ER and salt stresses. OsFes1C interacts directly with OsBiP1 and the interaction is inhibited by ATP but promoted by ADP, suggesting that OsFes1C acts as a potential NEF of OsBiP1 in vivo. Overexpression or suppression of OsFes1C led to hypersensitivity to ER stress and affected the growth of rice. Furthermore, we established that OsFes1C directly interacts with a putative salt response protein and is involved in the salt response. Taken together, our study marks an important step toward elucidating the functional mechanisms of an identified ER stress response factor in rice.
学科领域	Plant Sciences
DOI	10.1093/plphys/kiab263
收录类别	SCI
语种	英语
WOS关键词	PROTEIN-QUALITY CONTROL ; ENDOPLASMIC-RETICULUM ; PHOTOMORPHOGENIC DEVELOPMENT ; GENE-EXPRESSION ; FACTOR FES1 ; HSP70 ; RICE ; DEGRADATION ; ACCUMULATION ; STABILITY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000696244400037
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-2548
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences [XDA24010400] ; National Program of Transgenic Variety Development of China [2016ZX08001-006] ; Natural Science Foundation of China [31801267]
作者邮箱	lqqu@ibcas.ac.cn
作品OA属性	Green Published, Bronze
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26447
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Qian, Dandan,Xiong, Shuo,Li, Mei,et al. OsFes1C, a potential nucleotide exchange factor for OsBiP1, is involved in the ER and salt stress responses[J]. PLANT PHYSIOLOGY,2021,187(1):396-408.
APA	Qian, Dandan,Xiong, Shuo,Li, Mei,Tian, Lihong,&Qu, Le Qing.(2021).OsFes1C, a potential nucleotide exchange factor for OsBiP1, is involved in the ER and salt stress responses.PLANT PHYSIOLOGY,187(1),396-408.
MLA	Qian, Dandan,et al."OsFes1C, a potential nucleotide exchange factor for OsBiP1, is involved in the ER and salt stress responses".PLANT PHYSIOLOGY 187.1(2021):396-408.

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