SNARE proteins VAMP721 and VAMP722 mediate the post-Golgi trafficking required for auxin-mediated development in Arabidopsis

doi:10.1111/tpj.15450

	SNARE proteins VAMP721 and VAMP722 mediate the post-Golgi trafficking required for auxin-mediated development in Arabidopsis
	Zhang, Liang 1; Ma, Jingwen ; Liu, Huan ; Yi, Qian ; Wang, Yanan ; Xing, Jingjing 2; Zhang, Peipei ; Ji, Shengdong ; Li, Mingjun ; Li, Jingyuan ; Shen, Jinbo 3; Lin, Jinxing 1,4
	2021
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	108 期号:2 页码:426-440
摘要	The plant hormone auxin controls many aspects of plant development. Membrane trafficking processes, such as secretion, endocytosis and recycling, regulate the polar localization of auxin transporters in order to establish an auxin concentration gradient. Here, we investigate the function of the Arabidopsis thaliana R-SNAREs VESICLE-ASSOCIATED MEMBRANE PROTEIN 721 (VAMP721) and VAMP722 in the post-Golgi trafficking required for proper auxin distribution and seedling growth. We show that multiple growth phenotypes, such as cotyledon development, vein patterning and lateral root growth, were defective in the double homozygous vamp721 vamp722 mutant. Abnormal auxin distribution and root patterning were also observed in the mutant seedlings. Fluorescence imaging revealed that three auxin transporters, PIN-FORMED 1 (PIN1), PIN2 and AUXIN RESISTANT 1 (AUX1), aberrantly accumulate within the cytoplasm of the double mutant, impairing the polar localization at the plasma membrane (PM). Analysis of intracellular trafficking demonstrated the involvement of VAMP721 and VAMP722 in the endocytosis of FM4-64 and the secretion and recycling of the PIN2 transporter protein to the PM, but not its trafficking to the vacuole. Furthermore, vamp721 vamp722 mutant roots display enlarged trans-Golgi network (TGN) structures, as indicated by the subcellular localization of a variety of marker proteins and the ultrastructure observed using transmission electron microscopy. Thus, our results suggest that the R-SNAREs VAMP721 and VAMP722 mediate the post-Golgi trafficking of auxin transporters to the PM from the TGN subdomains, substantially contributing to plant growth.
关键词	auxin transporter SNARE post-Golgi trafficking trans-Golgi network Arabidopsis thaliana
学科领域	Plant Sciences
DOI	10.1111/tpj.15450
收录类别	SCI
语种	英语
WOS关键词	ARF-GEF ; PLASMA-MEMBRANE ; EFFLUX CARRIER ; TRANSPORT ; ENDOCYTOSIS ; NETWORK ; DOMAIN ; LOCALIZATION ; CONTRIBUTES ; RESISTANCE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000691434400001
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	National Natural Science Foundation of China [31770197, 32170339, 31300163, 31900162, 31970182] ; Program for Science & Technology Innovation Talents in the Universities of Henan Province [21HASTIT036] ; Natural Science Foundation of Henan Province [212300410049] ; Support Plan for Young Backbone Teacher in Colleges and Universities of Henan Province [2020GGJS067] ; Research Fund of Henan Normal University [2020JQ03] ; Young Elite Scientists Sponsorship Program by CAST [2019QNRC001]
作者邮箱	linjx@ibcas.ac.cn
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26451
专题	中科院北方资源植物重点实验室
作者单位	1.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Henan, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Henan Univ, Sch Life Sci, Key Lab Plant Stress Biol, Kaifeng 457001, Peoples R China 4.Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China 5.Beijing Forestry Univ, Coll Biol Sci Biotechnol, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Liang,Ma, Jingwen,Liu, Huan,et al. SNARE proteins VAMP721 and VAMP722 mediate the post-Golgi trafficking required for auxin-mediated development in Arabidopsis[J]. PLANT JOURNAL,2021,108(2):426-440.
APA	Zhang, Liang.,Ma, Jingwen.,Liu, Huan.,Yi, Qian.,Wang, Yanan.,...&Lin, Jinxing.(2021).SNARE proteins VAMP721 and VAMP722 mediate the post-Golgi trafficking required for auxin-mediated development in Arabidopsis.PLANT JOURNAL,108(2),426-440.
MLA	Zhang, Liang,et al."SNARE proteins VAMP721 and VAMP722 mediate the post-Golgi trafficking required for auxin-mediated development in Arabidopsis".PLANT JOURNAL 108.2(2021):426-440.

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