Carbon limitation overrides acidification in mediating soil microbial activity to nitrogen enrichment in a temperate grassland

doi:10.1111/gcb.15819

	Carbon limitation overrides acidification in mediating soil microbial activity to nitrogen enrichment in a temperate grassland
	Ning, Qiushi 1; Hattenschwiler, Stephan 2; Lu, Xiaotao 3; Kardol, Paul 4; Zhang, Yunhai; Wei, Cunzheng ; Xu, Chengyuan 5; Huang, Jianhui; Li, Ang ; Yang, Junjie ; Wang, Jing ; Peng, Yang ; Penuelas, Josep 6,7; Sardans, Jordi 6,7; He, Jizheng 8; Xu, Zhihong 1; Gao, Yingzhi 9; Han, Xingguo3,10
	2021
发表期刊	GLOBAL CHANGE BIOLOGY
ISSN	1354-1013
卷号	27 期号:22 页码:5976-5988
摘要	Higher ecosystem nitrogen (N) inputs resulting from human activities often suppress soil microbial biomass and respiration, thereby altering biogeochemical cycling. Soil acidification and carbon (C) limitation may drive these microbial responses, yet their relative importance remains elusive, which limits our understanding of the longer term effects of increasing N inputs. In a field experiment with continuous N addition at seven different rates from 0 to 50 g N m(-2) year(-1) over 6 years in a temperate grassland of Inner Mongolia, China, we examined the responses of soil microbial biomass and respiration to changes in soil acidity and C availability by adding lime and/or glucose to soil samples. Soil microbial biomass and respiration did only weakly respond to increasing soil pH, but increased strongly in response to higher C availability with increasing N addition rates. Soil net N immobilization increased in response to glucose addition, and soil microbial biomass increased at higher rates than microbial respiration along the gradient of previous N addition rates, both suggesting increasingly reinforced microbial C limitation with increasing N addition. Our results provide clear evidence for strong N-induced microbial C limitation, but only little support for soil acidity effects within the initial pH range of 4.73-7.86 covered by our study. Field data support this conclusion by showing reduced plant C allocation belowground in response to N addition, resulting in soil microbial C starvation over the long term. In conclusion, soil microbial biomass and respiration under N addition were strongly dependent on C availability, most likely originating from plant belowground C inputs, and was much less affected by changes in soil pH. Our data help clarify a long-standing debate about how increasing N input rates affect soil microbial biomass and respiration, and improve the mechanistic understanding of the linkages between ecosystem N enrichment and C cycling.
关键词	belowground carbon allocation carbon use efficiency microbial carbon starvation nitrogen deposition organic matter decomposition soil acidification
学科领域	Biodiversity Conservation ; Ecology ; Environmental Sciences
DOI	10.1111/gcb.15819
收录类别	SCI
语种	英语
WOS关键词	LONG-TERM NITROGEN ; ORGANIC-MATTER ; TERRESTRIAL ECOSYSTEMS ; AGRICULTURAL SOILS ; BACTERIAL-GROWTH ; BIOMASS ; DEPOSITION ; FOREST ; ROOTS ; AVAILABILITY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000685695900001
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-2486
资助机构	National Key RD Program [2016YFC0500700] ; Open Foundation of the State Key Laboratory of Urban and Regional Ecology of China [SKLURE2021-2-1] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences [2018032] ; Catalan Government [SGR2017-1005] ; Fundacion Ramon Areces ; European Research Council [ERCSyG-2013-610028 IMBALANCE-P]
作者邮箱	gaoyz108@nenu.edu.cn ; xghan@ibcas.ac.cn
引用统计	被引频次：53[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26478
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 2.Griffith Univ, Sch Environm & Sci, Nathan, Qld, Australia 3.Univ Montpellier, CNRS, EPHE, CEFE,IRD, Montpellier, France 4.Chinese Acad Sci, Inst Appl Ecol, Erguna Forest Steppe Ecotone Res Stn, Shenyang, Peoples R China 5.Swedish Univ Agr Sci, Dept Forest Ecol & Management, Umea, Sweden 6.Cent Queensland Univ, Sch Hlth Med & Appl Sci, Bundaberg, Qld, Australia 7.CREAF, Cerdanyola Del Valles, Catalonia, Spain 8.UAB, CSIC, CREAF, Global Ecol Unit, Bellaterra, Catalonia, Spain 9.Fujian Normal Univ, Key Lab Humid Subtrop Ecogeog Proc, Minist Educ, Fuzhou, Peoples R China 10.Northeast Normal Univ, Key Lab Vegetat Ecol, Changchun 130024, Peoples R China 11.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Ning, Qiushi,Hattenschwiler, Stephan,Lu, Xiaotao,et al. Carbon limitation overrides acidification in mediating soil microbial activity to nitrogen enrichment in a temperate grassland[J]. GLOBAL CHANGE BIOLOGY,2021,27(22):5976-5988.
APA	Ning, Qiushi.,Hattenschwiler, Stephan.,Lu, Xiaotao.,Kardol, Paul.,Zhang, Yunhai.,...&Han, Xingguo.(2021).Carbon limitation overrides acidification in mediating soil microbial activity to nitrogen enrichment in a temperate grassland.GLOBAL CHANGE BIOLOGY,27(22),5976-5988.
MLA	Ning, Qiushi,et al."Carbon limitation overrides acidification in mediating soil microbial activity to nitrogen enrichment in a temperate grassland".GLOBAL CHANGE BIOLOGY 27.22(2021):5976-5988.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Global Change Biolog（1840KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文