DEAD-BOX RNA HELICASE 27 regulates microRNA biogenesis, zygote division, and stem cell homeostasis

doi:10.1093/plcell/koaa001

	DEAD-BOX RNA HELICASE 27 regulates microRNA biogenesis, zygote division, and stem cell homeostasis
	Hou, Xiu-Li ; Chen, Wen-Qiang ; Hou, Yifeng 2,3; Gong, Hua-Qin ; Sun, Jing 2,3; Wang, Zhen 2,3; Zhao, Heng ; Cao, Xiaofeng 2,3; Song, Xiu-Fen ; Liu, Chun-Ming
	2021
发表期刊	PLANT CELL
ISSN	1040-4651
卷号	33 期号:1 页码:66-84
摘要	After double fertilization, zygotic embryogenesis initiates a new life cycle, and stem cell homeostasis in the shoot apical meristem (SAM) and root apical meristem (RAM) allows plants to produce new tissues and organs continuously. Here, we report that mutations in DEAD-BOX RNA HELICASE 27 (RH27) affect zygote division and stem cell homeostasis in Arabidopsis (Arabidopsis thaliana). The strong mutant allele rh27-1 caused a zygote-lethal phenotype, while the weak mutant allele rh27-2 led to minor defects in embryogenesis and severely compromised stem cell homeostasis in the SAM and RAM. RH27 is expressed in embryos from the zygote stage, and in both the SAM and RAM, and RH27 is a nucleuslocalized protein. The expression levels of genes related to stem cell homeostasis were elevated in rh27-2 plants, alongside down-regulation of their regulatory microRNAs (miRNAs). Further analyses of rh27-2 plants revealed reduced levels of a large subset of miRNAs and their pri-miRNAs in shoot apices and root tips. In addition, biochemical studies showed that RH27 associates with pri-miRNAs and interacts with miRNA-biogenesis components, including DAWDLE, HYPONASTIC LEAVES 1, and SERRATE. Therefore, we propose that RH27 is a component of the microprocessor complex and is critical for zygote division and stem cell homeostasis.
学科领域	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
DOI	10.1093/plcell/koaa001
收录类别	SCI
语种	英语
WOS关键词	ARABIDOPSIS-THALIANA ; PROTEIN INTERACTIONS ; FEEDBACK LOOP ; PRI-MIRNA ; SHOOT ; GENE ; EXPRESSION ; MERISTEM ; ENCODES ; CLV3
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000637288800009
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-298X
资助机构	National Transgenic Science and Technology Program [2019ZX08010-003] ; National Natural Science Foundation of China [31871455, 30625016, 31788103] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
作者邮箱	xfsong@ibcas.ac.cn ; cmliu@ibcas.ac.cn
作品OA属性	Green Published, Bronze
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26486
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Natl Ctr Plant Gene Res, CAS Ctr Excellence Mol Plant Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 5.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Hou, Xiu-Li,Chen, Wen-Qiang,Hou, Yifeng,et al. DEAD-BOX RNA HELICASE 27 regulates microRNA biogenesis, zygote division, and stem cell homeostasis[J]. PLANT CELL,2021,33(1):66-84.
APA	Hou, Xiu-Li.,Chen, Wen-Qiang.,Hou, Yifeng.,Gong, Hua-Qin.,Sun, Jing.,...&Liu, Chun-Ming.(2021).DEAD-BOX RNA HELICASE 27 regulates microRNA biogenesis, zygote division, and stem cell homeostasis.PLANT CELL,33(1),66-84.
MLA	Hou, Xiu-Li,et al."DEAD-BOX RNA HELICASE 27 regulates microRNA biogenesis, zygote division, and stem cell homeostasis".PLANT CELL 33.1(2021):66-84.

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