Knowledge Management System Of Institute Of Botany,CAS
Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes | |
Kimura, Y.; Yamashita, T.; Seto, R.; Imanishi, M.; Honda, M.; Nakagawa, S.; Saga, Y.; Takenaka, S.; Yu, L. -J.; Madigan, M. T.; Wang-Otomo, Z. -Y. | |
2021 | |
发表期刊 | PHOTOSYNTHESIS RESEARCH |
ISSN | 0166-8595 |
卷号 | 148期号:1-2页码:77-86 |
摘要 | The core light-harvesting complexes (LH1) in bacteriochlorophyll (BChl) b-containing purple phototrophic bacteria are characterized by a near-infrared absorption maximum around 1010 nm. The determinative cause for this ultra-redshift remains unclear. Here, we present results of circular dichroism (CD) and resonance Raman measurements on the purified LH1 complexes in a reaction center-associated form from a mesophilic and a thermophilic Blastochloris species. Both the LH1 complexes displayed purely positive CD signals for their Q(y) transitions, in contrast to those of BChl a-containing LH1 complexes. This may reflect differences in the conjugation system of the bacteriochlorin between BChl b and BChl a and/or the differences in the pigment organization between the BChl b- and BChl a-containing LH1 complexes. Resonance Raman spectroscopy revealed remarkably large redshifts of the Raman bands for the BChl b C3-acetyl group, indicating unusually strong hydrogen bonds formed with LH1 polypeptides, results that were verified by a published structure. A linear correlation was found between the redshift of the Raman band for the BChl C3-acetyl group and the change in LH1-Q(y) transition for all native BChl a- and BChl b-containing LH1 complexes examined. The strong hydrogen bonding and pi-pi interactions between BChl b and nearby aromatic residues in the LH1 polypeptides, along with the CD results, provide crucial insights into the spectral and structural origins for the ultra-redshift of the long-wavelength absorption maximum of BChl b-containing phototrophs. |
关键词 | Light-harvesting Reaction center Hydrogen bond π – π interaction Redshift ultra-redshift |
学科领域 | Plant Sciences |
DOI | 10.1007/s11120-021-00831-5 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | CORE COMPLEX |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000638054400001 |
出版者 | SPRINGER |
文献子类 | Article |
出版地 | DORDRECHT |
EISSN | 1573-5079 |
资助机构 | JSPS KAKENHI [JP16H04174, JP18H05153, JP20H05086, JP20H02856] ; Takeda Science Foundation ; Kurata Memorial Hitachi Science and Technology Foundation, Japan ; National Key R&D Program of China [2019YFA0904600] ; [19K06563] |
作者邮箱 | ykimura@people.kobe-u.ac.jp ; wang@ml.ibaraki.ac.jp |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/26504 |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.[Kimura, Y. 2.Seto, R. 3.Imanishi, M. 4.Takenaka, S.] Kobe Univ, Grad Sch Agr, Dept Agrobiosci, Nada Ku, Kobe, Hyogo 6578501, Japan 5.[Yamashita, T. 6.Honda, M. 7.Wang-Otomo, Z. -Y.] Ibaraki Univ, Fac Sci, Mito, Ibaraki 3108512, Japan 8.[Nakagawa, S. 9.Saga, Y.] Kindai Univ, Dept Chem, Higashiosaka, Osaka 5778502, Japan 10.[Yu, L. -J.] Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China 11.[Madigan, M. T.] Southern Illinois Univ, Dept Microbiol, Carbondale, IL 62901 USA |
推荐引用方式 GB/T 7714 | Kimura, Y.,Yamashita, T.,Seto, R.,et al. Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes[J]. PHOTOSYNTHESIS RESEARCH,2021,148(1-2):77-86. |
APA | Kimura, Y..,Yamashita, T..,Seto, R..,Imanishi, M..,Honda, M..,...&Wang-Otomo, Z. -Y..(2021).Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes.PHOTOSYNTHESIS RESEARCH,148(1-2),77-86. |
MLA | Kimura, Y.,et al."Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes".PHOTOSYNTHESIS RESEARCH 148.1-2(2021):77-86. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Kimura-2021-Circular(1733KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论