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Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes
Kimura, Y.; Yamashita, T.; Seto, R.; Imanishi, M.; Honda, M.; Nakagawa, S.; Saga, Y.; Takenaka, S.; Yu, L. -J.; Madigan, M. T.; Wang-Otomo, Z. -Y.
2021
发表期刊PHOTOSYNTHESIS RESEARCH
ISSN0166-8595
卷号148期号:1-2页码:77-86
摘要The core light-harvesting complexes (LH1) in bacteriochlorophyll (BChl) b-containing purple phototrophic bacteria are characterized by a near-infrared absorption maximum around 1010 nm. The determinative cause for this ultra-redshift remains unclear. Here, we present results of circular dichroism (CD) and resonance Raman measurements on the purified LH1 complexes in a reaction center-associated form from a mesophilic and a thermophilic Blastochloris species. Both the LH1 complexes displayed purely positive CD signals for their Q(y) transitions, in contrast to those of BChl a-containing LH1 complexes. This may reflect differences in the conjugation system of the bacteriochlorin between BChl b and BChl a and/or the differences in the pigment organization between the BChl b- and BChl a-containing LH1 complexes. Resonance Raman spectroscopy revealed remarkably large redshifts of the Raman bands for the BChl b C3-acetyl group, indicating unusually strong hydrogen bonds formed with LH1 polypeptides, results that were verified by a published structure. A linear correlation was found between the redshift of the Raman band for the BChl C3-acetyl group and the change in LH1-Q(y) transition for all native BChl a- and BChl b-containing LH1 complexes examined. The strong hydrogen bonding and pi-pi interactions between BChl b and nearby aromatic residues in the LH1 polypeptides, along with the CD results, provide crucial insights into the spectral and structural origins for the ultra-redshift of the long-wavelength absorption maximum of BChl b-containing phototrophs.
关键词Light-harvesting Reaction center Hydrogen bond π π interaction Redshift ultra-redshift
学科领域Plant Sciences
DOI10.1007/s11120-021-00831-5
收录类别SCI
语种英语
WOS关键词CORE COMPLEX
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000638054400001
出版者SPRINGER
文献子类Article
出版地DORDRECHT
EISSN1573-5079
资助机构JSPS KAKENHI [JP16H04174, JP18H05153, JP20H05086, JP20H02856] ; Takeda Science Foundation ; Kurata Memorial Hitachi Science and Technology Foundation, Japan ; National Key R&D Program of China [2019YFA0904600] ; [19K06563]
作者邮箱ykimura@people.kobe-u.ac.jp ; wang@ml.ibaraki.ac.jp
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/26504
专题中科院光生物学重点实验室
作者单位1.[Kimura, Y.
2.Seto, R.
3.Imanishi, M.
4.Takenaka, S.] Kobe Univ, Grad Sch Agr, Dept Agrobiosci, Nada Ku, Kobe, Hyogo 6578501, Japan
5.[Yamashita, T.
6.Honda, M.
7.Wang-Otomo, Z. -Y.] Ibaraki Univ, Fac Sci, Mito, Ibaraki 3108512, Japan
8.[Nakagawa, S.
9.Saga, Y.] Kindai Univ, Dept Chem, Higashiosaka, Osaka 5778502, Japan
10.[Yu, L. -J.] Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China
11.[Madigan, M. T.] Southern Illinois Univ, Dept Microbiol, Carbondale, IL 62901 USA
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GB/T 7714
Kimura, Y.,Yamashita, T.,Seto, R.,et al. Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes[J]. PHOTOSYNTHESIS RESEARCH,2021,148(1-2):77-86.
APA Kimura, Y..,Yamashita, T..,Seto, R..,Imanishi, M..,Honda, M..,...&Wang-Otomo, Z. -Y..(2021).Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes.PHOTOSYNTHESIS RESEARCH,148(1-2),77-86.
MLA Kimura, Y.,et al."Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes".PHOTOSYNTHESIS RESEARCH 148.1-2(2021):77-86.
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