Ectopic expression of the Arabidopsis florigen gene FLOWERING LOCUS T in seeds enhances seed dormancy via the GA and DOG1 pathways

doi:10.1111/tpj.15354

	Ectopic expression of the Arabidopsis florigen gene FLOWERING LOCUS T in seeds enhances seed dormancy via the GA and DOG1 pathways
	Chen, Fengying 1; Li, Yu 1; Li, Xiaoying ; Li, Wenlong 2; Xu, Jimei 1; Cao, Hong ; Wang, Zhi 3; Li, Yong 4; Soppe, Wim J. J.; Liu, Yongxiu
	2021
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	107 期号:3 页码:909-924
摘要	Ectopic expression of specific genes in seeds could be a tool for molecular design of crops to alter seed dormancy and germination, thereby improving production. Here, a seed-specific vector, 12S-pLEELA, was applied to study the roles of genes in Arabidopsis seeds. Transgenic lines containing FLOWERING LOCUS T (FT) driven by the 12S promoter exhibited significantly increased seed dormancy and earlier flowering. Mutated FT(Y85H) and TERMINAL FLOWER1 (TFL1) transgenic lines also showed increased seed dormancy but without altered flowering time. FT(Y85H) and TFL1 caused weaker seed dormancy enhancement compared to FT. The FT and TFL1 transgenic lines showed hypersensitivity to paclobutrazol, but not to abscisic acid in seed germination. The levels of bioactive gibberellin 3 (GA(3)) and GA(4) were significantly reduced, consistent with decreased expression of COPALYL DIPHOSPHATE SYNTHASE (CPS), KAURENE OXIDASE (KO), GIBBERELLIN 3-OXIDASE2 (GA3ox2), and GA20ox1 in p12S::FT lines. Exogenous GA(4+7) could recover the germination ability of FT transgenic lines. These results revealed that FT regulates GA biosynthesis. A genetic analysis indicated that the GA signaling regulator SPINDLY (SPY) is epistatic to FT in GA-mediated seed germination. Furthermore, DELAY OF GERMINATION1 (DOG1) showed significantly higher transcript levels in p12S::FT lines. Seed dormancy analysis of dog1-2 spy-3 p12S::FT-2 indicated that the combination of SPY and DOG1 is epistatic to FT in the regulation of dormancy. Overall, we showed that ectopic expression of FT and TFL1 in seeds enhances dormancy through affecting GA and DOG1 pathways.
关键词	seed dormancy FLOWERING LOCUS T TERMINAL FLOWER1 seed-specific expression gibberellin pathway DOG1 pathway
学科领域	Plant Sciences
DOI	10.1111/tpj.15354
收录类别	SCI
语种	英语
WOS关键词	ABSCISIC-ACID ; NEGATIVE REGULATOR ; GIBBERELLIN BIOSYNTHESIS ; NATURAL VARIATION ; GERMINATION ; PROTEIN ; ABA ; TEMPERATURE ; INDUCTION ; TIME
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000667128300001
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	National Natural Science Foundation of China [31801090, 31571257]
作者邮箱	yongxiu@ibcas.ac.cn
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26515
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 3.Sci & Technol Daily, Beijing, Peoples R China 4.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang, Peoples R China 5.Univ Freiburg, Inst Genet Epidemiol, Med Ctr, Freiburg, Germany 6.Soppe, Wim J. J.] Rijk Zwaan Breeding BV, De Lier, Netherlands
推荐引用方式 GB/T 7714	Chen, Fengying,Li, Yu,Li, Xiaoying,et al. Ectopic expression of the Arabidopsis florigen gene FLOWERING LOCUS T in seeds enhances seed dormancy via the GA and DOG1 pathways[J]. PLANT JOURNAL,2021,107(3):909-924.
APA	Chen, Fengying.,Li, Yu.,Li, Xiaoying.,Li, Wenlong.,Xu, Jimei.,...&Liu, Yongxiu.(2021).Ectopic expression of the Arabidopsis florigen gene FLOWERING LOCUS T in seeds enhances seed dormancy via the GA and DOG1 pathways.PLANT JOURNAL,107(3),909-924.
MLA	Chen, Fengying,et al."Ectopic expression of the Arabidopsis florigen gene FLOWERING LOCUS T in seeds enhances seed dormancy via the GA and DOG1 pathways".PLANT JOURNAL 107.3(2021):909-924.

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