Functional redox links between lumen thiol oxidoreductase1 and serine/threonine-protein kinase STN7

doi:10.1093/plphys/kiab091

IB-CAS > 中科院光生物学重点实验室

	Functional redox links between lumen thiol oxidoreductase1 and serine/threonine-protein kinase STN7
	Wu, Jianghao 1; Rong, Liwei 1; Lin, Weijun 1; Kong, Lingxi 1; Wei, Dengjie 1; Zhang, Lixin; Rochaix, Jean-David ; Xu, Xiumei 2
	2021
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	186 期号:2 页码:964-976
摘要	In response to changing light quantity and quality, photosynthetic organisms perform state transitions, a process which optimizes photosynthetic yield and mitigates photo-damage. The serine/threonine-protein kinase STN7 phosphorylates the light-harvesting complex of photosystem II (PSII; light-harvesting complex II), which then migrates from PSII to photosystem I (PSI), thereby rebalancing the light excitation energy between the photosystems and restoring the redox poise of the photosynthetic electron transport chain. Two conserved cysteines forming intra- or intermolecular disulfide bonds in the lumenal domain (LD) of STN7 are essential for the kinase activity although it is still unknown how activation of the kinase is regulated. In this study, we show lumen thiol oxidoreductase 1 (LTO1) is co-expressed with STN7 in Arabidopsis (Arabidopsis thaliana) and interacts with the LD of STN7 in vitro and in vivo. LTO1 contains thioredoxin (TRX)-like and vitamin K epoxide reductase domains which are related to the disulfide-bond formation system in bacteria. We further show that the TRX-like domain of LTO1 is able to oxidize the conserved lumenal cysteines of STN7 in vitro. In addition, loss of LTO1 affects the kinase activity of STN7 in Arabidopsis. Based on these results, we propose that LTO1 helps to maintain STN7 in an oxidized active state in state 2 through redox interactions between the lumenal cysteines of STN7 and LTO1.
学科领域	Plant Sciences
DOI	10.1093/plphys/kiab091
收录类别	SCI
语种	英语
WOS关键词	CYTOCHROME B(6)F COMPLEX ; STATE TRANSITIONS ; REDUCING EQUIVALENTS ; THYLAKOID MEMBRANE ; PHOTOSYSTEM-II ; SUBUNIT-IV ; PHOSPHORYLATION ; THIOREDOXIN ; IDENTIFICATION ; LTO1/ATVKOR
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000671555900021
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-2548
资助机构	National Key Research and Development Program [2020YFA0907600] ; National Natural Science Foundation of China [31700204, 31661143026] ; 111 Project [D16014] ; Outstanding Talents Fund of Henan University, China
作者邮箱	xuxiumei@vip.henu.edu.cn
作品OA属性	hybrid, Green Published
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26523
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Jinming Ave, Kaifeng 475004, Peoples R China 4.Univ Geneva, Dept Mol Biol, CH-1211 Geneva, Switzerland
推荐引用方式 GB/T 7714	Wu, Jianghao,Rong, Liwei,Lin, Weijun,et al. Functional redox links between lumen thiol oxidoreductase1 and serine/threonine-protein kinase STN7[J]. PLANT PHYSIOLOGY,2021,186(2):964-976.
APA	Wu, Jianghao.,Rong, Liwei.,Lin, Weijun.,Kong, Lingxi.,Wei, Dengjie.,...&Xu, Xiumei.(2021).Functional redox links between lumen thiol oxidoreductase1 and serine/threonine-protein kinase STN7.PLANT PHYSIOLOGY,186(2),964-976.
MLA	Wu, Jianghao,et al."Functional redox links between lumen thiol oxidoreductase1 and serine/threonine-protein kinase STN7".PLANT PHYSIOLOGY 186.2(2021):964-976.

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