N-6-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner

doi:10.1186/s13059-021-02385-0

	N-6-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner
	Zhou, Leilei 1; Tang, Renkun 1; Li, Xiaojing 1; Tian, Shiping1 ; Li, Bingbing 2; Qin, Guozheng1
	2021
发表期刊	GENOME BIOLOGY
ISSN	1474-760X
卷号	22 期号:1
摘要	Background Epigenetic mark such as DNA methylation plays pivotal roles in regulating ripening of both climacteric and non-climacteric fruits. However, it remains unclear whether mRNA m(6)A methylation, which has been shown to regulate ripening of the tomato, a typical climacteric fruit, is functionally conserved for ripening control among different types of fruits. Results Here we show that m(6)A methylation displays a dramatic change at ripening onset of strawberry, a classical non-climacteric fruit. The m(6)A modification in coding sequence (CDS) regions appears to be ripening-specific and tends to stabilize the mRNAs, whereas m(6)A around the stop codons and within the 3 ' untranslated regions is generally negatively correlated with the abundance of associated mRNAs. We identified thousands of transcripts with m(6)A hypermethylation in the CDS regions, including those of NCED5, ABAR, and AREB1 in the abscisic acid (ABA) biosynthesis and signaling pathway. We demonstrate that the methyltransferases MTA and MTB are indispensable for normal ripening of strawberry fruit, and MTA-mediated m(6)A modification promotes mRNA stability of NCED5 and AREB1, while facilitating translation of ABAR. Conclusion Our findings uncover that m(6)A methylation regulates ripening of the non-climacteric strawberry fruit by targeting the ABA pathway, which is distinct from that in the climacteric tomato fruit.
关键词	m(6)A methylation m(6)A methyltransferase Abscisic acid mRNA stability Translation efficiency Strawberry Fruit ripening
学科领域	Biotechnology & Applied Microbiology ; Genetics & Heredity
DOI	10.1186/s13059-021-02385-0
收录类别	SCI
语种	英语
WOS关键词	NUCLEAR-RNA ; NEGATIVE REGULATOR ; PROTEIN-KINASES ; CELL FATE ; METHYLATION ; REVEALS ; TRANSLATION ; DEMETHYLASE ; N6-METHYLADENOSINE ; RECEPTORS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000657355600001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Key Research and Development Program [2018YFD1000200] ; National Natural Science Foundation of China [31925035, 31930086, 31572174]
作者邮箱	gzqin@ibcas.ac.cn
作品OA属性	Green Submitted, Green Published, gold
引用统计	被引频次：68[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26527
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, 20 Nanxincun, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.China Agr Univ, Coll Hort, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Leilei,Tang, Renkun,Li, Xiaojing,et al. N-6-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner[J]. GENOME BIOLOGY,2021,22(1).
APA	Zhou, Leilei,Tang, Renkun,Li, Xiaojing,Tian, Shiping,Li, Bingbing,&Qin, Guozheng.(2021).N-6-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner.GENOME BIOLOGY,22(1).
MLA	Zhou, Leilei,et al."N-6-methyladenosine RNA modification regulates strawberry fruit ripening in an ABA-dependent manner".GENOME BIOLOGY 22.1(2021).

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