Genome Wide Analysis of GH Gene Family Reveals Vvgh9 Positively Regulates Sugar Accumulation under Low Sugar Content in Grape

doi:10.3390/horticulturae7110453

	Genome Wide Analysis of GH Gene Family Reveals Vvgh9 Positively Regulates Sugar Accumulation under Low Sugar Content in Grape
	Xu, Guangzhao 1; Wang, Yi ; Ren, Chong ; Fan, Peige ; Kuang, Yangfu 1; Wang, Yue 1; Liang, Zhenchang
	2021
发表期刊	HORTICULTURAE
卷号	7 期号:11
摘要	Sugar content directly affects grape (Vitis vinifera L.) berry quality and the resulting wine. Therefore, it is of great importance to study and explore novel genes that affect sugar accumulation in grapes. Glycosyl hydrolases (GHs) are key enzymes hydrolyzing polysaccharides into monosaccharides and play important roles in the regulation of carbohydrate metabolism. Nevertheless, the impact of GHs on the regulation of sugar accumulation in plants has rarely been investigated. In this study, we identified 11 putative GH genes in grapevines by phylogeny analysis. RNA-seq and quantitative real-time PCR results demonstrated that the expression level of VvGH9 was higher during the fruit set stage, which had lower sugar content than the veraison and ripe stages. Treatment of grape berries with exogenous sugar two weeks before veraison revealed that VvGH9 was rapidly induced by sucrose, fructose, and glucose. When '41B' calli was treated with different concentrations of glucose, VvGH9 expression increased at first and then decreased with the increase of glucose concentration. Overexpression of VvGH9 in grape calli and tomatoes also confirmed that this gene could contribute to sugar accumulation. All the above results demonstrated that VvGH9 promotes sugar accumulation under low sugar content in plants.
关键词	grape sugar VvGH9 transcriptomic analysis overexpression
学科领域	Horticulture
DOI	10.3390/horticulturae7110453
收录类别	SCI
语种	英语
WOS关键词	GLYCOSYL HYDROLASES ; STRESS ; IDENTIFICATION ; GLUCOSIDASE ; ENZYMES ; GROWTH
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000724360700001
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	2311-7524
资助机构	Strategic Priority Research Program of Chinese Academy of Sciences [XDA24030404-3] ; National Key Research and Development Program of China [2018YFD1000105] ; China-Africa Center for Research and Education, Chinese Academy of Sciences [151111KYSB20200017] ; Alliance of International Science Organizations [ANSO-CR-PP-2020-04]
作者邮箱	guangzhao90@163.com ; wangyi19881107@163.com ; chongr@ibcas.ac.cn ; fanpg@ibcas.ac.cn ; kuangyf@ibcas.ac.cn ; qiuyingwy@163.com ; zl249@ibcas.ac.cn
作品OA属性	gold
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26553
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Guangzhao,Wang, Yi,Ren, Chong,et al. Genome Wide Analysis of GH Gene Family Reveals Vvgh9 Positively Regulates Sugar Accumulation under Low Sugar Content in Grape[J]. HORTICULTURAE,2021,7(11).
APA	Xu, Guangzhao.,Wang, Yi.,Ren, Chong.,Fan, Peige.,Kuang, Yangfu.,...&Liang, Zhenchang.(2021).Genome Wide Analysis of GH Gene Family Reveals Vvgh9 Positively Regulates Sugar Accumulation under Low Sugar Content in Grape.HORTICULTURAE,7(11).
MLA	Xu, Guangzhao,et al."Genome Wide Analysis of GH Gene Family Reveals Vvgh9 Positively Regulates Sugar Accumulation under Low Sugar Content in Grape".HORTICULTURAE 7.11(2021).

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