Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063

doi:10.1038/s41477-021-00961-7

IB-CAS > 中科院光生物学重点实验室

	Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063
	Xiaol, Yanan 1; Huang, Guoqiang ; You, Xin ; Zhu, Qingjun 1; Wang, Wenda ; Kuang, Tingyun ; Han, Guangye ; Sui, Sen-Fang ; Shen, Jian-Ren
	2021
发表期刊	NATURE PLANTS
ISSN	2055-026X
卷号	7 期号:8 页码:1132-1142
摘要	Cryo-electron microscopy structures of two PSII intermediates from psbV-deletion and wild-type strains of cyanobacteria show substantial structural changes. These provide a structural basis for understanding the assembly process of native PSII. Photosystem II (PSII) is a multisubunit pigment-protein complex and catalyses light-induced water oxidation, leading to the conversion of light energy into chemical energy and the release of dioxygen. We analysed the structures of two Psb28-bound PSII intermediates, Psb28-RC47 and Psb28-PSII, purified from a psbV-deletion strain of the thermophilic cyanobacterium Thermosynechococcus vulcanus, using cryo-electron microscopy. Both Psb28-RC47 and Psb28-PSII bind one Psb28, one Tsl0063 and an unknown subunit. Psb28 is located at the cytoplasmic surface of PSII and interacts with D1, D2 and CP47, whereas Tsl0063 is a transmembrane subunit and binds at the side of CP47/PsbH. Substantial structural perturbations are observed at the acceptor side, which result in conformational changes of the quinone (Q(B)) and non-haem iron binding sites and thus may protect PSII from photodamage during assembly. These results provide a solid structural basis for understanding the assembly process of native PSII.
学科领域	Plant Sciences
DOI	10.1038/s41477-021-00961-7
收录类别	SCI
语种	英语
WOS关键词	CAB-LIKE PROTEINS ; SYNECHOCYSTIS-SP PCC-6803 ; CRYO-EM STRUCTURE ; SP PCC 6803 ; LIGHT-INDUCIBLE POLYPEPTIDES ; CRYSTAL-STRUCTURE ; WATER-OXIDATION ; CHLOROPHYLL ; COMPLEX ; OXYGEN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000669838400005
出版者	NATURE PORTFOLIO
文献子类	Article
出版地	BERLIN
EISSN	2055-0278
资助机构	National Key R&D Program of China [2017YFA0503700, 2016YFA0501101, 2017YFA0504600, 2020YFA0907600, 2019YFA0906300] ; National Natural Science Foundation of China [31470339] ; Strategic Priority Research Program of CAS [XDA27050402, XDB17000000] ; CAS Key Research programme for Frontier Science [QYZDY-SSW-SMC003] ; Youth Innovation Promotion Association of CAS [2020081] ; CAS Interdisciplinary Innovation Team [JCTD-2020-06]
作者邮箱	hanguangye@ibcas.ac.cn ; suisf@mail.tsinghua.edu.cn ; shen@cc.okayama-u.ac.jp
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26556
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Tsinghua Univ, Sch Life Sci, State Key Lab Membrane Biol, Beijing Adv Innovat Ctr Struct Biol, Beijing, Peoples R China 4.Tsinghua Univ, Frontier Res Ctr Biol Struct, Sch Life Sci, Beijing, Peoples R China 5.Southern Univ Sci & Technol, Dept Biol, Shenzhen, Peoples R China 6.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama, Japan 7.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama, Japan
推荐引用方式 GB/T 7714	Xiaol, Yanan,Huang, Guoqiang,You, Xin,et al. Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063[J]. NATURE PLANTS,2021,7(8):1132-1142.
APA	Xiaol, Yanan.,Huang, Guoqiang.,You, Xin.,Zhu, Qingjun.,Wang, Wenda.,...&Shen, Jian-Ren.(2021).Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063.NATURE PLANTS,7(8),1132-1142.
MLA	Xiaol, Yanan,et al."Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063".NATURE PLANTS 7.8(2021):1132-1142.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Xiaol-2021-Structura（5643KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文