Transcriptomic analysis of grapevine Dof transcription factor gene family in response to cold stress and functional analyses of the VaDof17d gene

doi:10.1007/s00425-021-03574-8

	Transcriptomic analysis of grapevine Dof transcription factor gene family in response to cold stress and functional analyses of the VaDof17d gene
	Wang, Zemin 1,2; Wang, Yi 1,2; Tong, Qian 1,2; Xu, Guangzhao 1,2; Xu, Meilong 1,2,5; Li, Huayang 1,2; Fan, Peige 1,4; Li, Shaohua1,2 ; Liang, Zhenchang 1,3
	2021
发表期刊	PLANTA
ISSN	0032-0935
卷号	253 期号:2
摘要	Main conclusionDof genes enhance cold tolerance in grapevine and VaDof17d is tightly associated with the cold-responsive pathway and with the raffinose family oligosaccharides.AbstractDNA-binding with one finger (Dof) proteins comprise a large family that plays important roles in the regulation of abiotic stresses. No in-depth analysis of Dof genes has been performed in the grapevine. In this study, we analyzed a total of 25 putative Dof genes in grapevine at genomic and transcriptomic levels, compiled expression profiles of 11 selected VaDof genes under cold stress and studied the potential function of the VaDof17d gene in grapevine calli. The 25 Dof proteins can be classified into four phylogenetic groups. RNA-seq and qRT-PCR results demonstrated that a total of 11 VaDof genes responded to cold stress. Comparative mRNA sequencing of 35S::VaDof17d grape calli showed that VaDof17d was tightly associated with the cold-responsive pathway and with the raffinose family oligosaccharides (RFOs), as observed by the up-regulation of galactinol synthase (GolS) and raffinose synthase genes. We found that the Dof17d-ED (CRISPR/Cas9-mediated mutagenesis of Dof17d-ED) mutant had low cold tolerance with a decreased RFOs level during cold stress. These results formed the fundamental knowledge for further analysis of the biological roles of Dof genes in the grapevine's adaption to cold stresses.
关键词	Cold stress CRISPR Cas9 Grapevine dof family Phylogenetic analysis Raffinose Transcriptomic analysis
学科领域	Plant Sciences
DOI	10.1007/s00425-021-03574-8
收录类别	SCI
语种	英语
WOS关键词	DNA-BINDING PROTEINS ; GENOME-WIDE ANALYSIS ; GALACTINOL SYNTHASE ; FREEZING TOLERANCE ; LOW-TEMPERATURE ; VITIS-VINIFERA ; PLANT ; EXPRESSION ; INVOLVEMENT ; OVEREXPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000616149400001
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1432-2048
资助机构	National Key Research and Development Program [2019YFD1002501] ; National Science Foundation of China [32025032]
作者邮箱	shhli@ibcas.ac.cn ; zl249@ibcas.ac.cn
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26565
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Innovat Acad Seed Design, Beijing 100093, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Plant Resources, Inst Bot, Innovat Acad Seed Design, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China 5.China Wine Ind Technol Inst, Yinchuan 750021, Ningxia, Peoples R China 6.State Key Lab Seedling Bioengn, Yinchuan 750004, Ningxia, Peoples R China
推荐引用方式 GB/T 7714	Wang, Zemin,Wang, Yi,Tong, Qian,et al. Transcriptomic analysis of grapevine Dof transcription factor gene family in response to cold stress and functional analyses of the VaDof17d gene[J]. PLANTA,2021,253(2).
APA	Wang, Zemin.,Wang, Yi.,Tong, Qian.,Xu, Guangzhao.,Xu, Meilong.,...&Liang, Zhenchang.(2021).Transcriptomic analysis of grapevine Dof transcription factor gene family in response to cold stress and functional analyses of the VaDof17d gene.PLANTA,253(2).
MLA	Wang, Zemin,et al."Transcriptomic analysis of grapevine Dof transcription factor gene family in response to cold stress and functional analyses of the VaDof17d gene".PLANTA 253.2(2021).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Wang-2021-Transcript（2357KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文